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TP350 User’s Manual

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2.01

Содержание PCR TP350

Страница 1: ...IO INC Attention Users are recommended to carefully read the contents of this manual before operating the TaKaRa PCR Thermal Cycler DiceTM Touch Carefully observe all special Warnings and Cautions out...

Страница 2: ...g foreign counterparts thereto L45 Heated Cover This product is covered by the claims of U S Patent 5 552 580 and their corresponding foreign counterpart patent claims Thermal Cycler DiceTM is a trade...

Страница 3: ...nd Their Functions 11 Chapter 4 System Operation 13 1 Introduction 13 2 PCR Profile Creation 15 3 PCR File Manager 22 4 Manual Incubation 24 5 Configuration Tools 25 Chaptur 5 Diagnosis Functions 29 1...

Страница 4: ...nsibilities Refer to the Warrantycard included with this product for detailed description of the Warranty conditions and period This product is designed to ultimate safety standards Nevertheless shoul...

Страница 5: ...internal overheating that may result in physical burns or fire Avoid installing the system in locations subject to dust or strong vibrations Avoid installing in locations subject to direct sunlight a...

Страница 6: ...ng the system Other precautions Observe the following precautions in order to prevent electric shocks and fires Avoid inserting metal objects flammable materials or other foreign objects into the vari...

Страница 7: ...fires Grounding Mandatory Power cable Mise la Terre Obligatoire Cordon d alimentation Always ground the system to prevent electric shock from leakage current Burns High Temperatures Heat cover Heat bl...

Страница 8: ...copied without the express permission of the copyright holder Copyright holder TAKARA BIO INC Printed in Japan Contact TAKARA BIO INC TAKARA BIO INC SETA3 4 1 OTSU SHIGA 520 2193 JAPAN Tel 81 77 543...

Страница 9: ...g system configured and optimized for industry standard 0 2ml individual or strip tubes domed or flat capped or 96 well plates Applications of thermal gradient technology gradient of between 0 25 12 s...

Страница 10: ...of heating and cooling between 0 1 sec 2 5 sec during a run Manual Control mode enables the user to set the block to a specific temperature quickly without creating a thermal profile This function is...

Страница 11: ...ar equipment that generate noise caused by electrical motors and transformers 6 Choose a flat location capable of withstanding the design weight of the system 7 The installation environment must be wi...

Страница 12: ...1 Thoroughly clean the system and make sure that there are no tubes or plates remaining in the sample block 2 Avoid any shock during movement that can affect the operation of this highly sophisticated...

Страница 13: ...ting fingers as the Top Heater interior of the unit reaches very high temperature up to 115 in operation Touch Panel 7 inch vivid color touch screen controls the program at your tip of your finger or...

Страница 14: ...which sample tubes and plates are placed Caution Do not touch the block as it reaches temperatures up to 99 Power Switch The Power Switch is main switch to put on or shut off the power Power Cable Con...

Страница 15: ...will display the logo and take a few moments to initiate the software Caution Please do not touch the Touch panel and shut down the system until the main menu appear on the panel screen That is the ca...

Страница 16: ...vide access the following features PCR Program Editor is used to create a new PCR thermal profile and initiate its execution PCR File Manager is used to load and edit PCR program files Manual Incubati...

Страница 17: ...a stored program on the device Add Step Add a step file to specify the location of the displayed profile Add to end of Profile Add a step to end of the displayed profile Insert after X Add a step clo...

Страница 18: ...ck hat icon worn Wizard under the screen and open the default profile Change a block temperature to click a temperature column Change a reaction time to click a time column Change a number of cycles t...

Страница 19: ...rresponding check boxes Each of the steps will be executed in the order they are shown arranged down the screen with the denaturation Anneal and Extension if enabled steps being cycled After the ampli...

Страница 20: ...ple hold at given temperature for a set time It may be performed either with or without a gradient across the block If you wish to hold the temperature infinity you may touch the Infinite button Ramp...

Страница 21: ...gressively increased or decreased from an initial value to a final value over a defined number of steps Long Range allows the hold time of a given step within a cycling event to be progressively incre...

Страница 22: ...kbox option is selected default then you will be prompted with a screen asking if you wish to wait for the top heater or reach target temperature before commencing the run If you select to wait for th...

Страница 23: ...use and stop using right icons on the Run Screen On run completion the post run report screen will show and if enabled an audible alarm will sound The alarm may be muted by pressing the mute alarm but...

Страница 24: ...around plural target files by a stylus and then select Copy icon on the lower side of screen Paste Appear a Paste icon when select a Copy icon on the lower side of screen Paste copied files for other...

Страница 25: ...er s Manual PostRunReport The post run report can be saved as a PostRunReport text file on the PCR File Management maximum recent 10 files Specification is described content of a PostRunReport file v...

Страница 26: ...ight sides of the block with a roughly linear thermal gradient presented between the two outer well sides and the predicted well temperatures can be shown by clicking the View Gradient Temp link Top H...

Страница 27: ...er recalibration for the life of the machine The recalibration procedure is not covered in this user manual If it is required contact the support line service of Takara Bio The thermal calibration is...

Страница 28: ...the users area you are able to select an existing user or create or delete a user profile When a user is selected thermal profiles will be loaded or saved to a directory specific to that user Logging...

Страница 29: ...Most manufacturers thermal cyclers purely use the block temperature for timing Accelerated Thermal Equilibration If this feature is selected the temperature model will use overshoot and undershoot to...

Страница 30: ...the software which resides on the Takara Thermal cycler DiceTM Touch The upgrade file you receive from our support team should be placed on the main root directory of your USB memory stick You may the...

Страница 31: ...t at room temperature prior to commencing test The Diagnosis function performs the following three tests 1 Cycle Test From the home screen the PCR File Manager icon will bring to the experiment page Y...

Страница 32: ...efore confirming commencement and then click Start Run The Program is started The below report is described to the end of Cycle Test program Run Started Run completed and Run duration is appeared The...

Страница 33: ...st 3p file to preset program in Dice and appear the specification of this program at the right side of PCR File Manager Touch a Edit File icon show the content of program Program details 98 for 1min 2...

Страница 34: ...bed to the end of Up Down Test program Run Started Run completed and Run duration is appeared The report is saved a PostRunReport file You checked that the run durations less than the Standard Value T...

Страница 35: ...Select the Ramp Rate Test t3p file to preset program in Dice and click the checkmark Touch a Edit File icon show the content of program Program details 25 for 30sec 95 for 30sec 40 for 30sec 1 cycle...

Страница 36: ...P350 User s Manual The Program is started The below report is described to the end of Ramp Rate Test program Click the lower right check then the Configuration is selected from the home screen v2 01 3...

Страница 37: ...on the Calibration Wizard screen The Max Cool rate and Max Heat rate is displayed on the System Massage Reference Value Max Cool is more than 2 5 sec Max Heat is more than 3 0 sec After ramp rate is c...

Страница 38: ...e Top Heater module Need to replace the Top Heater Module PCB fault Need to replace the Main Module Touch screen does not work but when a mouse is plugged in it works and the touch screen cannot be re...

Страница 39: ...power switch and take off the power cord for safety 2 Warn to spill neither water nor a reagent to mechanism parts and electric parts Moreover please place neither detergent nor medicine on equipment...

Страница 40: ...e When a heated top heater becomes by power failure below in a predetermined temperature resume operation after warming a heated top heater to an appropriate temperature The program will be ended and...

Страница 41: ...l Memory 256MB enough for 10 000 saved profiles USB Flash memory is enable to use Heated Top Heater range Controllable 60 115 Internal Interface Embedded graphical controller with 7 widescreen touch s...

Страница 42: ...tylus Touch pen 1 4 Warranty sheet 1 5 TaKaRa Supplied PCR Tubes trial set Single Tube 0 2 ml Hi Tube Dome Cap Single Tube Cat NJ200 100 8 Tube Cap 0 2 ml Hi 8 Tube 8 Tube Cat NJ300 12 0 2 ml Hi 8 Fla...

Страница 43: ...8 Cap NJ300 0 2 ml Hi 8 Tube 8 Tube 125 NJ301 0 2 ml Hi 8 Dome Cap 125 NJ302 0 2 ml Hi 8 Flat Cap 8 cap 125 NJ600 0 2 ml 8 strip tube individual Flat Caps 120 0 2 ml Plate Cap NJ710 96 well snap plat...

Страница 44: ...athogenicity none possibly yes Toxicity none possibly yes Radioactive substances not used used nuclide 2 System contamination status no contamination decontamination possibly contaminated This is to c...

Страница 45: ..._________________________________________________________________________ ______________________________________________________________________________________________________________________________...

Страница 46: ...SETA3 4 1 OTSU SHIGA 520 2193 URL http www takara bio com 2015 03 09...

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