CONTROLLED COPY
Centre for Biological Engineering
Standard Operating Procedure
SOP122
Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK
Location:
CBE Laboratory Unit (H21/22)
Version 003
Effective Date: 26
th
June 2018
Review 26
th
June 2020
Written by: Victoria Workman
Reviewed by: P.Hourd
Approved by: R/I.Temple
Page 9 of 22
(vi) To change the time in steps of 1 second (instead of in steps of 10 seconds in the min:sec mode)
or in steps of 1 minute (instead of in steps of 10 minutes in the hrs:min mode): Keep the stop key
pressed while setting the desired runtime with the right rotary knob.
(vii) To run in Short Run or Continuous Run mode see the user manual
(viii) It is possible to change the runtime whilst the centrifuge is running
5.3.3 Setting the speed
(i) To preselect a speed turn the left rotary knob until “set” appears in front of the parameter “Speed”
on the display.
(ii) Select the option by pressing or turning the right rotary knob. “Set” will now flash.
(iii) Turn the right rotary knob until the desired value is displayed.
(iv) Press the right rotary knob to confirm the selected value. If this is not done, the value will be
automatically reset to the last setting.
(v) When the maximum permissible speed of the rotor is reached, the “max” display lights up.
(vi) It is possible to change the speed during the centrifugation run.
(vii) To change the speed in steps of 1 rpm (instead of in steps of 100 rpm): Keep the stop key
pressed while setting the desired speed with the right rotary knob.
5.3.4 Setting the RCF value
(i) To preselect the RCF value turn the left rotary knob until “set” appears in front of the parameter
“rcf” on the display.
(ii) Select the option by pressing or turning the right rotary knob. “Set” will now flash.
(iii) Turn the right rotary knob until the desired value is displayed.
(iv) Press the right rotary knob to confirm the selected value. If this is not done, the value will be
automatically reset to the last setting.
(v) When the maximum permissible RCF value of the rotor is reached, the “max” display lights up.
(vi) It is possible to change the RCF value during the centrifugation run.
(vii) To change the speed in steps of 1 x g (instead of in steps of 10 x g): Keep the stop key pressed
while setting the desired RCF value with the right rotary knob.
