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Sigma 147161, Руководство по эксплуатации


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Refrigerated Centrifuge with Heating System Sigma 4-16KHS 

  

 

11 Appendix 

 

 

 

82 / 91 

Version 10/2012, Rev. 1.22 of 08/05/2020 

• sb 

Translation of the original operating manual, part no. 0701802 

 

-  no data 
1 resistant 
2 practically resistant 
3 partially resistant 
4 not resistant 
 

  

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Medium 

Formula 

 [%

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PE

 

PA

 

 PC

 

PO

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PP

 

PSU

 

PVC

 

PVC

 

PTF

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NBR

 

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Sodium bisulphite 

NaHSO

3

 

10  1 

1 

2 

4 

1 

- 

- 

- 

1 

1 

1 

Sodium carbonate 

Na

2

CO

3

 

10  1 

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1 

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Sodium chloride 

NaCl 

30  1 

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1 

1 

1 

1 

1 

1 

1 

1 

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Sodium hydroxide 

NaOH 

30  1 

1 

4 

1 

1 

1 

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2 

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Sodium hydroxide 

NaOH 

50  1 

1 

4 

1 

1 

1 

1 

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1 

2 

4 

Sodium sulfate 

Na

2

SO

4

 

10  1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

Spirits 

C

2

H

6

O 

96  1 

1 

1 

1 

1 

1 

1 

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1 

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1 

Styrene 

C

8

H

8

 

100  4 

1 

4 

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Sulphuric acid 

H

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4

 

6  1 

4 

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1 

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1 

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1 

2 

3 

Sulphuric acid 

H

2

SO

4

 

fuming  4 

4 

4 

4 

4 

4 

4 

4 

1 

4 

3 

Tallow 

— 

100  1 

1 

1 

1 

1 

- 

1 

1 

1 

1 

1 

Tetrahydrofuran (THF)  C

4

H

8

O 

100  3 

1 

4 

1 

3 

4 

4 

4 

1 

3 

1 

Tetrahydronaphthalene  C

10

H

12

 

100  3 

1 

4 

1 

4 

4 

4 

4 

1 

- 

1 

Thionyl chloride 

Cl

2

SO 

100  4 

4 

4 

2 

4 

4 

4 

4 

1 

- 

3 

Tin chloride 

SnCl

2

 

10  1 

4 

2 

2 

1 

- 

- 

- 

1 

1 

4 

Transformer oil 

— 

100  1 

1 

3 

3 

1 

1 

1 

- 

1 

1 

1 

Trichloroethane 

C

2

H

3

Cl

3

 

100  3 

3 

4 

2 

4 

4 

4 

4 

1 

4 

4 

Urea 

CH

4

N

2

O 

10  1 

1 

1 

1 

1 

- 

- 

- 

1 

1 

1 

Urine 

— 

100  1 

1 

1 

1 

1 

- 

1 

1 

1 

- 

2 

Vinegar 

C

2

H

4

O

2

 

10  1 

4 

1 

1 

1 

1 

1 

1 

1 

2 

1 

Vinegar 

C

2

H

4

O

2

 

90  1 

4 

4 

4 

1 

3 

1 

4 

1 

- 

1 

Wax 

— 

100 

- 

1 

1 

  

1 

- 

- 

- 

1 

- 

1 

Wines 

— 

100  1 

1 

1 

2 

1 

1 

1 

1 

1 

- 

4 

Xylene 

C

8

H

10

 

100  3 

1 

4 

1 

4 

4 

4 

4 

1 

4 

1 

 

Pos :  229  /010 Univ ers almodul e/ Seit enwechs el  @  0\mod_1202116244312_0.docx  @  105 @   @  1 

 

 

Содержание 147161

Страница 1: ...e original operating manual sb 10 2012 Rev 1 22 of 08 05 2020 Sigma 4 16KHS part no 0701802 Sigma 4 16KHS from serial no 147161 Refrigerated Centrifuge with Heating System Operating Manual Please reta...

Страница 2: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 2 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Страница 3: ...of 08 05 2020 sb 3 91 Translation of the original operating manual part no 0701802 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 F...

Страница 4: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 4 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Страница 5: ...2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions...

Страница 6: ...of a rotor 29 6 2 2 2 Installation of an angle rotor with a hermetic lid 30 6 2 2 3 Installation of accessories 32 6 2 2 4 Adapters 34 6 2 2 5 Vessels 34 6 3 Control system Spincontrol S 35 6 3 1 Use...

Страница 7: ...8 1 3 Rotors buckets and carriers 64 8 1 4 Load bearing bolts 65 8 1 5 Glass breakage 66 8 2 Sterilisation and disinfection of the rotor chamber and accessories 66 8 2 1 Autoclaving 67 8 3 Service 68...

Страница 8: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS Table of contents 8 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Страница 9: ...a Standardm odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separ...

Страница 10: ...dmodule 010 Allgemei ne Informati onen 010 0050 N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declarat...

Страница 11: ...n Pos 16 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 020 Aufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 10...

Страница 12: ..._4 16KH S 020 Aufbau und Wirkungsw eise 020 0010 0020 Typenschild 4 16KHS 29 mod_1405326892357_68 docx 198625 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5...

Страница 13: ...cally as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor...

Страница 14: ...rifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram prov...

Страница 15: ...1378 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name pl...

Страница 16: ...es and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life en...

Страница 17: ...y The centrifuge has to be maintained regularly see chapter 8 Maintenance and service Components that are not in a perfect state must be replaced immediately Pos 37 010 U niversalmodule Leerzeile 0 m...

Страница 18: ...g in compliance with the relevant standards Pos 44 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 45 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0...

Страница 19: ..._0 docx 114 1 Pos 49 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 m od_1405319028141_68 docx 192101 3 1 3 6 4 Chemical a...

Страница 20: ...bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installati...

Страница 21: ...ion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service lif...

Страница 22: ...bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing furt...

Страница 23: ...og box see chapter 7 2 Table of error codes Pos 66 010 U niversalmodule Leerzeile 8pt 12 mod_1337063062114_0 docx 65687 1 Pos 67 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Страница 24: ...gen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in co...

Страница 25: ...m od_1405319161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage tem...

Страница 26: ...Lagerung und Tr ansport 040 0050 Transportsicherung 4 16KS_KHS berschrift 29 mod_1405326914634_68 docx 198709 2 1 4 5 Transport safety device Pos 90 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 4 16K...

Страница 27: ...s condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the centrifuge can be used again Pos 95 010 U niversalmodule Leerzeile 0 m...

Страница 28: ...eits au er 3 30KS 7 mod_1306312302397_68 docx 43513 3 1 5 2 2 Customer provided fuses Typically the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer NOTE T...

Страница 29: ...1405319202508_68 docx 192465 3 1 6 2 1 Opening and closing the lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the l...

Страница 30: ...x 114 1 Pos 113 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S 060 0020 0020 0023 Einsetzen eines Wi nkelrotors mit H ermetikdeckel 10 Nm 29 m od_1405326759765_68 do...

Страница 31: ...original operating manual part no 0701802 Loosen the rotor tie down screw anti clockwise and remove the rotor together with the lid Loosen the lid by turning it anti clockwise and then remove it 1 Ro...

Страница 32: ...ation of accessories Only use vessels that are suitable for the rotor In the case of swing out rotors all of the compartments must be equipped with buckets Always load rotationally symmetrical compart...

Страница 33: ...loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 9 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations N...

Страница 34: ...S 060 0020 0020 0051 Gef e 4 16S 4 16KS 4 16KHS 56 mod_1521541840863_68 docx 559986 4 1 6 2 2 5 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or multiple carriers cause cor...

Страница 35: ...mod_1405320193718_68 docx 193123 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several d...

Страница 36: ...ifuge lid must be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is t...

Страница 37: ...he actual speed is displayed below this value The values are stated in revolutions per minute min 1 rpm and they depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal forc...

Страница 38: ...peed is reached Select the field Runtime and press the function knob The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 cloc...

Страница 39: ...unction knob in order to accept the data Fig 14 Rotor selection list here for a swing out rotor Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being...

Страница 40: ...Standard menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode...

Страница 41: ...No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message...

Страница 42: ...runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 17 Progress indicator during a centrifugation run After the...

Страница 43: ...me and temperature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be display...

Страница 44: ...still Depending on the substances that are to be centrifuged it may make sense to bring the centrifuge to a certain temperature prior to starting a centrifugation The temperature adaptation prevents t...

Страница 45: ...ward facing arrow in the speed field Values between 1 2 and 10 0 g cm3 are possible Centrifugation monitoring The centrifugation monitoring function enables the continuous monitoring of the speed and...

Страница 46: ...ed if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message wil...

Страница 47: ...he Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock...

Страница 48: ...ge It enables the optimum adaptation of the centrifuge to its specific area of application Fig 22 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens w...

Страница 49: ...erature can be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follo...

Страница 50: ...e entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next cha...

Страница 51: ...u The curve editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used...

Страница 52: ...e since the necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary...

Страница 53: ...BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Programmbetri eb 27 m od_1405320235093_68 docx 193459 3 1 6 3 3 Program mode A program contains a...

Страница 54: ...to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be...

Страница 55: ...list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and...

Страница 56: ...n in the Setup menu Fig 29 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the co...

Страница 57: ...ding on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 160 010 Universalmodul e Leerzeile 0 mod_12021162445...

Страница 58: ...nnection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact servic...

Страница 59: ...he opening on the left side of the control panel e g with a screwdriver Fig 31 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole T...

Страница 60: ...centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance err...

Страница 61: ...ent of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma z...

Страница 62: ...al stress e g by heat generators Pos 175 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 176 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080...

Страница 63: ...m odul e 080 War tung und Instandhaltung 080 0010 0020 Zubeh r au er 8K 28 m od_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must...

Страница 64: ...elfachtr ger au er Basic 28 mod_1405320621063_68 docx 194873 3 1 8 1 3 Rotors buckets and carriers Rotors buckets and carriers are produced with the highest precision in order to withstand the perman...

Страница 65: ...t of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bol...

Страница 66: ...Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the gre...

Страница 67: ...Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 3...

Страница 68: ...o withstand this high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especial...

Страница 69: ...ment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and sp...

Страница 70: ...afe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new pack...

Страница 71: ...ermissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of dec...

Страница 72: ...requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Input fuse AT 2 0 16 0 at 220 240 V 50 Hz temperature fuses Performance data Max speed rpm Max capacit...

Страница 73: ...y of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx...

Страница 74: ...ith a data sheet that includes important information and notes on safety This data sheet must be added to the operating manual Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 P...

Страница 75: ...l operating manual part no 0701802 Pos 222 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed...

Страница 76: ...ope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maxim...

Страница 77: ...Appendix Version 10 2012 Rev 1 22 of 08 05 2020 sb 77 91 Translation of the original operating manual part no 0701802 Fig 39 Diagram of linear curves Fig 40 Diagram of quadratic curves Pos 225 010 Uni...

Страница 78: ...dition 1 16K Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 1...

Страница 79: ...1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Alumini...

Страница 80: ...iaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylanili...

Страница 81: ...2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1...

Страница 82: ...30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric...

Страница 83: ...v 1 22 of 08 05 2020 sb 83 91 Translation of the original operating manual part no 0701802 Pos 230 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 4 16KS_4 16KHS 110 Anhang 110 0060 Konformit tserkl r un...

Страница 84: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 11 Appendix 84 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Страница 85: ...d Centrifuge with Heating System Sigma 4 16KHS 11 Appendix Version 10 2012 Rev 1 22 of 08 05 2020 sb 85 91 Translation of the original operating manual part no 0701802 11 7 Declaration of conformity C...

Страница 86: ...ated Centrifuge with Heating System Sigma 4 16KHS 11 Appendix 86 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802 Ende der Liste f r Textm arke...

Страница 87: ...trifugation monitoring 45 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with low capacity 33 Centrifugation with vessels of vari...

Страница 88: ...he symbols and notes 16 Explosive substances 19 External signal 50 F Filling quantity refrigerant 72 Fire preventions 19 Form for the return of defective parts 69 Function 48 Functional and operating...

Страница 89: ...n mode active 36 Motor error 60 Motor shaft 29 30 62 Multiple carrier 34 N Name plate 11 12 No indication on the display 58 No power in the mains supply 58 Noise level 72 Nominal voltage 12 Notes on s...

Страница 90: ...instructions for centrifugation 20 Safety of rotors and accessories 21 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 54 Scope of...

Страница 91: ...r chamber 23 Temperature monitoring 46 Temperature monitoring system 23 Temperature range 72 Temperature unit 49 Temperature value not reached 58 Thermal stress 62 Time fine 49 Time range 72 Toxic sub...

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