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XFp Extracellular Flux Analyzer User Manual

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Table of Contents

1. XFp Analyzer Overview

............................................................................................................... 4

2. Technical Specifications

.............................................................................................................. 5

3. XFp Analyzer Installation

  Unpacking and Component Identification ................................................................................... 6
  Suitable Locations for the XFp Analyzer ....................................................................................... 7
    Safety Considerations ................................................................................................................... 7

4. Basic Operation of the XFp Analyzer

  Power and Warm-Up .................................................................................................................... 8
  Welcome Screen on First Time Power Up .................................................................................... 8
  Communications and Network Connection Setup ....................................................................... 8
  Turning the XFp Analyzer Off ....................................................................................................... 8

5. Navigating the XFp Analyzer software

  Setting up an XFp Assay................................................................................................................ 9
  Running an XFp Assay ................................................................................................................. 11
  Modifying XFp Analyzer Settings ................................................................................................ 14
  XFp Analyzer Diagnostics ............................................................................................................ 16
  Online Help and Support ............................................................................................................ 18
  Analyzing XFp Data Using Wave for Desktop  ............................................................................ 19

Appendices

  Appendix I: Networking Guide ................................................................................................... 20
  Appendix II: Troubleshooting ..................................................................................................... 24
  Appendix III: Support Contact and Ordering Information .......................................................... 25

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Содержание XFp

Страница 1: ...XFp Extracellular Flux Analyzer User Manual Part 102745 400 Rev A...

Страница 2: ...d N Billerica MA 01862 Submit technical support questions or comments about our documentation to support seahorsebio com Phone support Global United States United States Toll Free United Kingdom Germa...

Страница 3: ...ty Considerations 7 4 Basic Operation of the XFp Analyzer Power and Warm Up 8 Welcome Screen on First Time Power Up 8 Communications and Network Connection Setup 8 Turning the XFp Analyzer Off 8 5 Nav...

Страница 4: ...concentration drops approximately 20 30 and media pH declines approximately 0 1 0 2 pH units The measurement is performed using optical fluorescent biosensors embedded in a disposable cartridge that i...

Страница 5: ...43 cm x 58 cm Weight 33 lbs 15 Kg Power Requirements 100 240V AC 50 60Hz 6A 3A Power cord rating 3 wire grounded AC power cord rated 10A or greater Power fuse ratings 250V 5A Time Lag 2 fuses 5mm X 20...

Страница 6: ...on Unpacking and Component Identification Upon receipt immediately check each box for damage Report any shipping damage to the transportation company and Seahorse Bioscience using the contact informat...

Страница 7: ...vers be securely attached and plate tray door is closed This also prevents heat loss and system cooling which can affect data quality The door opens automatically when the tray is extended allowing th...

Страница 8: ...Navigating the XFp Analyzer Software XFp Analyzer Communications and Network Connection Setup The transfer of template and result files between the XFp Analyzer and an analysis computer running Wave c...

Страница 9: ...pear and allow selection and running of a template from the networked location ii If a USB thumb drive containing a valid XFp template has been inserted into the instrument a USB tab will appear Templ...

Страница 10: ...uide for XFp Analyzer for more information about measurement cycles 4 Review Summary and Start Assay a Review the General Information and Errors and Warnings sections to verify that the settings are a...

Страница 11: ...Calibration of the sensor cartridge which takes approximately 20 minutes Note Although the sensor calibration may appear to be complete after 15 minutes the instrument will be busy for a few more minu...

Страница 12: ...uching the Options button different data can be displayed 1 Show StdDev turns on display of error bars for the each Group 2 Level Data shows the concentration data from which the rate data is derived...

Страница 13: ...te 7 After the assay is complete the data will automatically be saved to a USB flash drive if inserted the local drive or the networked drive if configured A message will appear indicating the locatio...

Страница 14: ...which the instrument is located 2 Backup Assay Results a Backup Assay Results saves a copy of each assay performed within the previous 60 days on the local storage within the XFp Analyzer i Insert a U...

Страница 15: ...work access The Ethernet cable port is located on the lower backside of the XFp Analyzer b Wireless Network A wireless USB adapter can be plugged into one of the available USB ports recommended on low...

Страница 16: ...Overall System Communication Test Verifies all system modules are communicating appropriately Voltage Test Ensures voltage levels are within specified ranges for operation Motor Test Verifies the move...

Страница 17: ...XFp Analyzer is designed to require minimal cleaning and maintenance All consumables are disposable and none of the instrument components are exposed to the cell plate at any time preventing cross con...

Страница 18: ...ion is contained on the Help screen 1 Support Worldwide Seahorse Support contact information 2 Version of Software Hardware Currently installed software and firmware versions on XFp Analyzer If the XF...

Страница 19: ...nd installed on any personal computer via the Seahorse Bioscience website http www seahorsebio com support software update php Refer to the Wave User Guide for XFp Seahorse Document 103244 400 for a d...

Страница 20: ...internal Wi Fi adapter in the instrument The Ethernet RJ 45 jack is located at base of the instrument in the back The XFp Analyzer can be connected to any Microsoft Windows compatible network and the...

Страница 21: ...al IP Address Assignment 1 Remove the green check from the Obtain IP Address Automatically check box 2 Manually enter the information provided by the local IT department for the instrument 3 After set...

Страница 22: ...To Setup 10 Click the Ping button in the Wired Network Connection Settings screen 11 If there is a Pass message and icon the wireless setup is complete a If it fails confirm the information in the wir...

Страница 23: ...t can be automatically set Time Zone Setup 1 From the Home screen click Settings and then click Go To Setup 2 Press the right arrow at the bottom right four times The Time Zone tab will appear 3 Selec...

Страница 24: ...ail result delete the contents of every field on this page and click Apply The templates page should now be displayed quickly Instrument gives a load error message and it is not clear whether a cartri...

Страница 25: ...ates Toll Free 800 671 0633 option 3 Global United States 1 978 671 1600 option 3 United Kingdom 0800 096 7632 Germany 0800 180 6678 Europe 45 31 36 98 78 Consumables Order To order XFp FluxPaks inclu...

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