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whilst passing through the heat exchanger bubbles are minimized. In order for this
to work the solution should be held in a vessel closed to air and supplied with
95/5% O2/CO2 reservoir. The diagram below shows a typical vessel setup. In this
case the vessel is securely mounted at the desired height and the head of pressure
is determined by the height of the lower end of the central glass tube. This ensures
that the flow rate remains constant throughout the entire experiment.
Setting up the vacuum.
The bath solution height is maintained and adjusted using the vacuum wicking
method. The SliceMate bath has a pair of oval suction vents which are connected
to the outlet port at the rear of the SliceMate. A typical vacuum/waste setup uses
either house vacuum or a suitable pump which is connected to a waste container,
which is in turn connected to the outlet port at the rear of the SliceMate.
The wick is made from filter paper which is cut into the shape shown below. The
wick is cut with a “V” at the one end of it. As the wick is moved back and forth
over the oval outlet ports the level of suction can be varied. This allows the user to
control the meniscus level above the bath.