Section 8
8
37
Research Instruments Ltd
Biopsy Mode
Biopsy Mode is available with the Saturn 5 Active Laser System only and can be used for assisted hatching
and embryo biopsy procedures. It allows the user to deliver a series of 4-6 shots along a predetermined
line automatically and has no behavioral differences to a succession of single shots; that is, it is as safe
as single shot mode. This predetermined line can be straight or curved.
When using this mode care must be taken
•
not to draw a line where you do not want a hole to be drilled
•
not to move the sample while firing the laser
•
to check the laser settings and alignment prior to firing
•
to fire in between two cells so that the cells are less exposed to the heat generated and to reduce
the chance of cell lysis and other cells around this vicinity (above or below) being also affected by
the heat.
•
gulations
How to Enable Biopsy Mode
1.
Click the
General Settings
icon on the tool bar.
2.
Tick
Enable Biopsy Mode.
(T
his option is available only with Saturn 5 Active Laser System).
How to Fire the Laser (Biopsy Mode)
1.
Switch to the laser objective.
2. Focus on the sample.
3. Click the
Laser Control
Icon on the tool bar.
4.
Check the sample is just on the bottom of the dish.
5.
From the Laser Control Panel, click
Biopsy
. You will be taken to the Laser Biopsy panel.
6.
Click where you want the first hole to be. The line will follow your cursor.
7.
Click where you want the last hole to be. The holes to be drilled are overlaid on the image.
8. The size and number of holes can be changed by moving the two sliders on the panel.
9.
The line can be lengthened or shortened by dragging the line end.
10.
A point in the middle of the line can be dragged to create a curved line.
11.
Press
Start Firing
to begin the firing procedure. The laser will then drill each hole in sequence.
If Biopsy Mode needs to be stopped at any stage press
Stop.
How to Shut Down
1.
Close RI Viewer software.
2.
Unplug the device from the power outlet.
