Section 8
Saturn 5™ Laser Operation
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Research Instruments Ltd
How to Adjust Hole Size/Pulse Width
1.
Hold the left mouse button down and drag until the desired size is reached (Fixed laser systems only).
2.
For Active systems the option to click and drag can be turned off in the
General Settings.
3. Click and drag the slider on the
Laser Control
panel.
4.
Fine adjustment can be made using the
up down
keys.
Preset Pulse Widths
Preset pulse widths are 0.350
ms
, 0.400
ms and 0.450ms and
these preset values can be changed using RI Viewer.
Choose the preset by clicking the
+
or
-
buttons.
To change a preset, select the preset, set the desired value using
the slider, and click the
S
ave
button.
Hole sizes can also be chosen by clicking on the screen and
dragging a hole of the size you need. The pulse width will be
calculated automatically to create the size of hole required.
How to Fire the Laser (Single Pulse)
Find the sample under the microscope using an objective with wide field of view (for example the 4x
objective).
1. Hold the sample with a holding pipette at the bottom of the dish.
2. Switch to the laser objective and focus on the sample.
3. Click the
Laser Control
icon on the tool bar.
4.a Fixed System - position the sample such that the desired hole location is under the target.
4.b Active System - position the sample in the field of view. Click on the desired position of the hole.
5. Click the
Fire
button. The control unit will beep to confirm that the laser has fired
In some installations, the field of view is larger than the range of movement of the laser. If the cursor is
moved to a position on the screen where the laser cannot reach, the unreachable area will be highlighted.
The target cannot be placed in this area.
