Section 7
Laser Ablation Procedure
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Research Instruments Ltd
For further information on the methodology of laser ablation, refer to the many published scientific
papers on the subject, or seek advice from a practitioner who is experienced in the technique.
Do not attempt to make a large hole with just one shot of the laser. Although this will
not cause damage to the instruments, it could potentially damage the embryo due to the
greater heating effect of the long pulse width.
Using a Saturn 5 Fixed Laser System, move the specimen into
the field of view. Select a short pulse width using
the RI Viewer
controls. The different settings do not vary the power of the
laser; they control the length of time that the laser is fired. If
using a Saturn 5 Active Laser System move the target on the
screen by clicking the position required with the mouse button.
Click on the zona pellucida where you want the hole to appear.
F
ire the laser by clicking the
Fire
button in RI Viewer. A small hole
should appear in the zona. If a larger hole is required, increase
the laser pulse time and fire again.
To create larger holes and for zona thinning the area should be
ablated using a series of small diameter holes that overlap slightly,
joining to form one larger hole or channel.
Incorrect focus for drilling.
