RI SATURN 5, Руководство пользователя

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Section 8
8
35
Saturn 5™ Laser Operation
Research Instruments Ltd
6. Click the
Save Point
button when you are happy that the sizes match.
7. Repeat for a number of pulse widths to create a curve on the graph.
How to Remove a Point from the Hole Size Calibration Graph
1. Click the point you wish to remove.
2. Click the Remove button.
The warning will disappear as soon as the negative gradient has been resolved.
Laser Target
RI Viewer displays a laser target on the image. This indicates where the laser beam is focused, and thus
where the hole will appear.
Note:
To use the laser system, the specimen must be viewed with the red laser objective.
The target will only be shown when the system is in laser mode.
The appearance and size of the laser target can be modified. The Target Appearance Setting is displayed
in the Laser Settings panel. You can change its appearance by adjusting the sliders. This is also where
the size of the laser Exclusion Zone can be set.
Hole Size Indicator
The circle that is displayed indicates the estimated size of the hole that will be created when the laser
is fired. Actual hole sizes may vary due to natural differences between specimens.
The Hole Size Indicator can be turned on or off from the Target Appearance panel on the Laser Settings
panel.
Exclusion Zone
The laser generates heat which has the potential to damage critical cells. The Exclusion Zone provides
a measuring tool to show whether critical cells are close to the laser ablation area and therefore may
be affected by the heat. The target point should be as far away from these critical cells as is possible to
avoid unnecessary damage and it is ultimately the responsibility of the user to define a safe distance.
The default exclusion zone setting is 5μm. This can be adjusted via the Target Appearance panel using
the slider or input box.
Repeated hole size calibration can sometimes introduce incorrect points on the graph.
The graph should normally be a straight line or smooth curve with a positive (increasing)
gradient. If a point leads to a negative gradient then a warning will be displayed on the
chart. This should be corrected as it will imply two different pulse widths for a given hole
size, and may lead to unexpected hole sizes.