XCell™ C410:V3 Controller
User Guide
8 repligen.com UG-C410v3-06
Figure 1. Filtration assembly connection to C410:V3 Controller and a bioreactor side port
1.1
XCell ATF® Device pump cycle
The diaphragm pump is the heart of the XCell ATF® Device process. It produces an alternating flow
through the HFM (lumen side) or SM. The XCell ATF® Device provides a pulsating, reversible, flow of
liquid, back and forth, between the process vessel and the diaphragm pump. The following is a
description of that process:
The diaphragm pump is partitioned into two chambers with a flexible diaphragm,
. One of
the pump chambers, the
pump liquid
(PL)
chamber
is connected to the Filter Housing, which, in
turn, is connected to the process vessel. Therefore, any flow between the diaphragm pump and
process vessel will be through the filtration device. The second pump chamber, the
pump air
(PA)
chamber,
is connected to the pump flow control system. Typically, controlled addition of
compressed air into the PA chamber increases the pressure in the chamber relative to the process
vessel, forcing the flexible diaphragm partitioning the two chambers to move into the PL chamber
and towards the vessel. Liquid in the PL chamber is forced through the filter to the process vessel.
The flow through the HFM (lumen side) generates tangential flow in one direction. This pumping
phase (or cycle) is called the pressure cycle. Inversely, with a pressurized process vessel relative to
PA or PL, or with an external vacuum supply, liquid will flow in the reverse direction, from process
vessel, through the HFM (lumen side), to the PL chamber, generating tangential flow in the other
direction. This pumping phase (or cycle) is called the exhaust cycle. These alternating pump cycles
are then repeated continuously. See also