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XCell™ C410:V3 Controller
User Guide
38 repligen.com UG-C410v3-06
Figure 21. Instrument flow control schematic of XCell™ C410:V3 Controller
The XCell ATF® Device flow control is achieved by regulating the pneumatic air flow to and from
the diaphragm pump; the pneumatic flow control is achieved with a two-stage control, by
regulating its pressure and with a flow restrictor. Two proportional pressure regulators valves,
PRV1 and PRV2, are designed to make fine adjustments in pressure to the air stream flowing from
the manual pressure regulator to a flow restrictor. Two automated flow restrictors, proportional
flow control valves, PV1 and PV2, are designed to make coarse adjustments in flow. Final flow
control is achieved by Step changes in PV orifice opening in combination with fine adjustments in
the air flow stream pressure with the PRV.
Adjustments in flow are based on the error difference between Calculated CT and Actual CT. The
proportional air pressure regulating valve, PRV1, and the exhaust pressure regulating valve, PRV2,
will be adjusted by the PLC based on the Error. The error will cause pressures to be changed to
affect the flow, positive or negative, respectively, to and from the pump to match flow set point
for the next cycle.
If the new value for PRV1 and/or PRV2 exceed their set pressure limits, (e.g. PRV1 0 to 30 psi, PRV2 0
to -14.5 psi), then, the respective PV1 and PV2 will adjust incrementally, (e.g. by user defined
increments (in the Basic Set Up screen)), until the PRVs are back within operational range.
3.11
Start-up
Assembly of the XCell ATF® Device is described in the following sections. This section provides a
startup guide for an XCell ATF® Device connected to a bioreactor and ready for use, with the
following general conditions:
•
An XCell ATF® 6 Device is used in this example.
•
An XCell ATF® Device flow of 12L/min is required.
•
Bioreactor pressure is 0.0 psi
•
∆
height between pump midpoint and vessel liquid level is 0 cm
•
Regulated air pressure set to 35 psi
•
Vacuum source connected
Following Log On and entry of Batch Information, go to the Set-up screen.
Go to the Basic Set Up screen, and enter the following field values:
SOL1
INCOMING AIR
EXHAUST (VACUUM)
PROPORTIONAL VALVE
PV1
PRESSURE
SENSOR
P2
TO PUMP
PROPORTIONAL VALVE
PV2
AUTOMATIC
PRESSURE
REGULATOR
PRV1
MANUAL
PRESSURE
REGULATOR
AUTOMATIC
VACUUM
REGULATOR
PRV2