Reichert Univar, Руководство пользователя

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B7
Transmitted-light microscopy with polarized light:
(A) with halogen lamp
⚪ Turn on halogen lamp with control transformer
⚪ Turn on relay (red dot)
⚪ Turn off Bertrand lens (pull out pin)
⚪ Turn off neutral density filter in the ocular body (black
dot not visible)
⚫ Insert analyzer and set to 0
⚪ Disengage the compensator
⚪ Pull out interference contrast main prism until it stops,
after loosening the clamping screw
⚫ Rotate to the 10x objective. Stress-free lenses must be
used for polarization-optical investigations
⚪ Switch off the exciter filter for transmitted-light (red
dot)
⚪ Switch off the neutral density filters for transmitted-
light (red dot)
⚫ Line up the brightfield condenser. The brightfield
condensers described on page B2 are available in strain-
free versions (P). Turn lower condenser turret to the empty
opening (red dot)
⚫ Turn condenser fine feed to the central position and
raise condenser with coarse feed up to the stop
⚪ Set the collector for transmitted-light halogen lamp (red
dot)
⚪ Switch off the auxiliary optics for wide-field condenser
(red dot)
⚪ Set the photo system magnification changer to low
magnification (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Set beam at 20% to the body (red dot, CAM/PRO)
⚪ Set the phase ring knob to empty position (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚪ On mirror housing 2, set rotary prism for transmitted-
light with halogen lamp
⚪ Switch off color filters for contrast fluorescence (red
dot)
⚪ On mirror housing 2, set excitation filter to red dot
⚪ Set sliding mirror to halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Close field iris diaphragm, then focus its image with
condenser fine adjustment
⚫ Center field iris diaphragm in the middle of the field
with centering screws. Then open field iris diaphragm just
larger than the field of view
⚫ Swivel the polarizer into place. The interference colors
are visible when observing a birefringent specimen
⚫ Close the condenser iris diaphragm to the usual
2/3-open position, highlighting the interference colors
⚪ For comparative observations with normal light you can
switch out the analyzer or the polarizer