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Operation
Ensure that there are no air bubbles under your samples.
As with any Sonicator application, the amplitude setting is subjective and may vary with each
user’s particular application. Most users have found their best results while running the MPH
at or above amplitude setting 5. It is recommended to start optimizing your procedure at low
settings and increase amplitude as needed.
If you are experiencing atomization from the microplate wells, cover with a lid or seal with
plastic film type cover. The sealing film is especially advantageous for microplates with wells
filled to their maximum level.
If you are sonicating for long time intervals, the horn and water will increase in temperature.
You should test the water by touch after each sonication interval is complete. If the water is
too warm, replace it with ice cold water. Programming the Sonicator to “pulse” mode will also
aid in keeping the temperature of the horn and the water cool. Circulating cold water through
the reservoir is highly recommended
All sonication times are application dependent. Always start at low amplitude settings and
short time frames. Increase your processing times and amplitude settings as necessary until
you achieve your desired results. The optimum scenario is to re-circulate ice-cold water with
pulsed sonication.
Maintenance
To ensure optimum performance it is highly recommended to remove the horn from the
converter and clean the mating surfaces on a regular basis. The horn and converter are
joined by a threaded stud. The vibration of the device causes stress and friction inside this
connection. Disassemble the unit with the wrench set, cleaning the threading and hole with a
cotton swab dipped in alcohol. Wipe the flat mating surfaces with alcohol. Let the parts air
dry and reassemble tightly with the wrench set. This preventive maintenance should be
done weekly.
The top surface of the MPH can be cleaned with a mild soapy solution and rinsed. Do not
use solvents or abrasives to clean the microplate horn. Do not allow water to drip into the
Converter (located below the horn). Water can damage the Converter and cause down time.
Water damage is not covered by the warranty.
Important Note
: When the unit is not in use, drain water and dry the surface of the horn.
The Horn is made of aluminum, and with use, you will notice a blackening or staining of the
surface of the horn. This is due to normal oxidation of the aluminum. Oxidation stains may
be apparent on brand new units since each unit is tested before it is shipped. This
staining/blackening has no effect on the performance of the Horn and the stain may
eventually fade (but not completely go away) over time.
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