Qsonica SONICATOR S-4000, Руководство по эксплуатации, Страница: 41 / 55

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14. Instructions for Specialty Horns
Cup Horn Instructions
1. Ensure rubber splash shield is securely placed under the cup.
2. Install and hand tighten the cup horn onto the converter. Then tighten the horn with the
pin spanner wrenches.
3. The Sound Enclosure holds the Cup Horn securely. See Sound Enclosure instructions for
details. If you do not have a Sound Enclosure, a strong lab stand using two clamps on
the
converter case
can be used. See page 13 if using a clamp.
4. Install fittings (plugs and/or hose adapters) to inlet, outlet and overflow ports. Teflon tape
may be used if fittings leak. Check alignment of O-rings to make sure they are straight.
5. Water should be filled up to the midpoint between the top of the horn and top of the
acrylic cup. Please allow room for displacement when sample is added.
For constant flow cooling, use hose adapters with soft tubing. Provide pinch clamps on the
inlet and outlet tubes to control flow. An open-bath re-circulating chiller is recommended for
maximum cooling. The flow required is about 500 ml/min. Place a flat plate on the cup to
avoid splashing if the coolant flow is turned too high.
For static cold water use, close off all three ports with the plugs provided and fill the cup. If
sonication is brief, the heat generated in the cup is insignificant to affect the sample. If the
water temperature rises, pour it out and refill the cup with cold water.
Another method of operation is to fit the outlet port with the hose adapter, soft tubing and a
pinch clamp. Run the tubing, without bends or loops, directly to a drain. Add ice and water
to the cup. After the ice melts, drain the cup and refill with ice and water. If the fittings leak,
place Teflon tape around the fitting threads. If coolant splashes into the shield, tip the shield
edge to drain it.
If you decide to use ice, it must be on the sides of the cup and not below your samples.
Acoustic energy travels throughout the area above the horn. The presence of ice can block
the transmission of energy, reducing its effectiveness.
For maximum transmission of acoustic energy into the sample vessel, use a cavitation-
resistant fluid such as a water-glycol mixture, pure ethylene or propylene glycol. The
sonication intensity will be greatly increased, in proportion to the glycol concentration. If
using tap water, you should degas the water before adding your samples.
Cup Horn Operation
Cup Horns are high intensity ultrasonic water baths that allow samples to be processed in
completely closed containers (such as test tubes or vials). The ultrasonic probe never comes
in contact with sample so that sample loss, escape and/or cross contamination cannot occur.
Larger Cup Horns allow for the simultaneous sonication of clusters of tube or vials providing
for uniformity of processing. Cup Horns are ideal for sterile or pathogenic sample