Qsonica SONICATOR S-4000 Скачать руководство пользователя страница 49 | Manualshive

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Suggested Microplates, Pumps and Chillers and where to buy them:

Microtiter Plates

96 shallow-well microplate:

•

96-well Full Skirt PCR plate, 2ml, part #285096. Manufacturer: E&K Scientific (1-800-934-8114)

•

Hard Shell™ Skirted Microplate, 96-well, part # HSP-9601. Manufacturer: MJ Research (1-888-
729-2165)

•

96-well Skirted Plate, part # 652270. Manufacturer: Greiner Bio-one (1-800-884-4703)

•

Thermal Cycler Plate, 0.2ml, part # UP 2312. Manufacturer: United Laboratory Plastics (1-800-
722-2499)

•

96-23II reaction plate, part # N801-0560. Manufacturer: Applied Biosystems (1-800-327-3002)

384-well microplate:

•

384-well Polypropylene, small volume, part # 785201. Manufacturer: Greiner Bio-one (1-800-884-
4703)

1536-well microplate:

•

1536-well Polypropylene, part #782270P. Manufacturer: Greiner Bio-one (1-800-884-4703)

Deep-well microplate (the first two are the most recommended since they have vents):

•

96-well Masterblock Deep-well, part #780201. Manufacturer: Greiner BioOne (1-800-884-4703)

•

Cluster Tube System, 1.2ml tubes, part #4412. Manufacturer: Corning (1-800-492-1110)

•

Assay Block, 96-well, 0.5ml PP, part #3957. Manufacturer: Corning/Costar (1-800-492-1110)

•

Assay Block, 96-well, 1ml PP, part #3959. Manufacturer: Corning/Costar (1-800-492-1110)

Chillers & Peristaltic Pumps

Thermo Fisher Catalog Chillers:

•

Catalog #13-874-7, Model 3006, capacity 6L

•

Catalog #22-037-157, Model WK-230 (Benchtop chiller), capacity 6L

Thermo Fisher Peristaltic Pumps:

•

Watson-Marlow PumpPro, Catalog #14-283-2 (Standard), Catalog #14-283-3 (Economy), NOTE:
ask for two pump heads

•

Manostat “Carter” Multichannel Pumps, Catalog #13-875-241. NOTE: ask for 2 channels that will
fit size 16 tubing or 1/8” ID tubing.

Cole Parmer Catalog Chillers:

•

High-Stability Digital Bath, Catalog #A-12108-00 (Analog), #A-12108-01(Standard), #A-12108-20
(Digital), capacity 6L

•

Julabo Refrigerated Circulating Baths, Catalog #

EW-15002-00

(Economy), Catalog

EW-15002-0

4

(Digital), capacity 4.5L

Cole Parmer Catalog Peristaltic Pumps:

•

Pump Heads (need 2) – Easy Load Pump Heads, Catalog #A-07518-00 for CRS rotor, PSF
housing, Catalog #A-07518-10 for SS rotor, PSF housing, Catalog #A-07518-60 for SS rotor, PPS
housing

•

Pump Heads (need 2) – Easy Load II Pump Heads, Catalog #A-077200-50 for CRS rotor, PSF
housing, Catalog #A-077200-60 for SS rotor, PSF housing, Catalog #A-077201-60 for SS rotor,
PPS housing

•

Pump Head (need 1) – Two channel Easy-Load II Pump Head, Catalog #A-77202-50 for CSR
rotor, Catalog #A-77202-60

•

Pump Drive – L/S Variable speed Economy Console Drive, Catalog #A-07554-80, 7 to 200rpm For
the peristaltic pumps you’ll need to use size 16 tubing or 1/8” ID tubing.

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Содержание SONICATOR S-4000

Страница 1: ...SONICATOR ULTRASONIC PROCESSOR Part No S 4000 OPERATION MANUAL 53 Church Hill Road Newtown CT 06470 203 426 0101 tel 203 426 7026 fax info sonicator com www sonicator com...

Страница 2: ...e Getting Started 11 14 6 Operating Instructions 15 24 7 Accessories 25 26 8 Sound Reducing Enclosure 27 29 9 Techniques for Optimizing Results 30 10 Maintenance 31 36 11 Troubleshooting 37 38 12 Warr...

Страница 3: ...d the manual in its entirety The new digital Sonicator offers several new and innovative features This manual provides the necessary instruction and guidance to help ensure the successful operation of...

Страница 4: ...es in temperature and humidity Do not place the Generator within a Fume Hood NEVER immerse the converter in liquids of any kind or let condensed moisture or liquid drip into the converter NEVER grasp...

Страница 5: ...C 120 VAC 8 Amps Fast Acting For 220 VAC 240 VAC 4 Amps Fast Acting Weight 13 5 lbs 6 1Kg Dimensions 11 W x19 5 L x 5 25 H 27 9cm x 49 5cm x 13 3cm Output Voltage 1000 V rms max Output Frequency 20 KH...

Страница 6: ...djustment See Option menu The Power Cord supplied with the Sonicator must be used If the 220V plug is not configured to match the wall receptacle a properly grounded universal AC socket adapter must b...

Страница 7: ...the cavitation field The erosion and shock effect of the collapse of the cavitation bubble is the primary mechanism of fluid processing The probe tip diameter dictates the amount of sample that can be...

Страница 8: ...1 100 giving a greater degree of resolution for setting the amplitude needed to effectively process samples Reference information such as converter power output joules and process time is also displa...

Страница 9: ...9 The S 4000 includes a standard diameter tapped horn 200 Generator Converter Horn with replaceable tip Converter Cable...

Страница 10: ...10 S 4000 Rear Panel On Off Switch Power Cord Connector Converter Cable Connector USB Connector Temperature Probe Connector Footswitch Jack Cooling Fan...

Страница 11: ...at the factory Check the tightness of the horn and flat tip by using the wrench set Please refer to images on page 12 of this manual A loose horn or tip may cause damage to the generator circuitry or...

Страница 12: ...Horn Tightening Tip Removal Tip Tightening Microtip Removal Microtip Tightening Wrench Set Note When tightening a Microtip the tip must not be in contact with the worksurface Always have the tip exte...

Страница 13: ...secure the connectors If a foot switch or timer switch is used plug it into the foot switch connector Make sure that the plug is inserted properly Converter and horn mounted in Sound Enclosure 432B P...

Страница 14: ...iquids solvents require a solid tip horn Set up regular preventive maintenance schedule See the Maintenance section for details Hearing protection is highly recommended The Sound Enclosure 432B reduce...

Страница 15: ...anually performed b Program Mode Allows the user to program specific On Off times at any programmed output setting The ability to save up to 10 programs and sequence several individual programs togeth...

Страница 16: ...then appears When using a Microtip the YES button must be selected Warning Improper selection will result in tip damage or poor sonication Damage caused by not following this step is not covered under...

Страница 17: ...s the user to create a program select or run a program and select or run a sequence of programs Option Screen The Option menu allows for the selection of Temperature units Footswitch operational modes...

Страница 18: ...intensity setting for your sample by touching the Up or Down arrow Touch Start to begin sonication Touch Pause to pause sonication Manual and Program modes both show the Start Pause and Exit buttons...

Страница 19: ...erent programs Select Modify Sequence 1 2 Select a sequence of programs for the unit to run in succession A maximum of 6 programs can be sequenced at one time Option Screen The Option menu enables the...

Страница 20: ...red the unit will run continuously without pulsing Process Time Pulse On and Pulse Off times are measured in Hours Minutes Seconds format 6 Activate Temperature Monitoring if necessary 7 Program a Shu...

Страница 21: ...n progress The Temperature Monitoring and Shutdown Temperature option have been selected during this example program Program Progress is displayed by a bar graph This displays the progress of the curr...

Страница 22: ...in the PGM fields 3 If a delay or rest time between programs is desired touch the Delay After field Enter the appropriate time frame 4 Unused PGM fields should have a dash Select unsused programs tou...

Страница 23: ...must be in the liquid sample If the initial sample temperature is above the set point sonication will not turn on During sonication if the temperature reaches the Shutdown value the unit will pause so...

Страница 24: ...g on the type of fault detected Below are two examples Further details are included in the Troubleshooting section The self diagnostic features of this unit apply to all standard horns and Microtips D...

Страница 25: ...ates the amount of sample that can be effectively processed Smaller tip diameters Microtip probes deliver high intensity sonication but the energy is focused within a small concentrated area Larger ti...

Страница 26: ...sels at one time Indirect sonication is most effective for very small samples because foaming and sample loss are eliminated Pathogenic or sterile samples are ideal for this method because aerosols an...

Страница 27: ...installation of horn Note A diameter rod is mounted inside the enclosure A standard laboratory clamp can be used to hold samples if necessary A clamp is not included with the enclosure For shipping p...

Страница 28: ...e assembly into the holder making sure the cable is not crimped There is a capped port on either side of the enclosure for cold water circulation during sonication See Section 14 for Cup Horn instruct...

Страница 29: ...d converter must be properly tightened using the appropriate wrench set before being inserted into the sound enclosure Remove collar and insert covertor as shown Attach collar around Converter and sec...

Страница 30: ...as a Cup Horn or Microplate Horn may be a better option Viscosity Limitations Viscous solutions and highly concentrated liquids can be difficult to sonicate If the liquid is so thick that it will not...

Страница 31: ...dure done with the Sonicator power switch in the OFF position and with the unit unplugged from the AC power source Long exposure to acids or caustics results in corrosion of metal parts or components...

Страница 32: ...eneral use the cable assembly should not be used to carry the converter or pull it toward the user Make certain the cable always has slack and is never tensioned If necessary move the generator or con...

Страница 33: ...33 Clean threaded Stud Clean threading in converter Do not allow liquid to drip into Convertor Allow parts to dry Tighten Stud to horn...

Страница 34: ...34 Cleaning horn stud Cleaning horn threading...

Страница 35: ...d turn the power on for a few seconds to remove residue The tip also can be sterilized using alcohol with the power on Alcohol is a volatile compound and proper handling precautions must be taken whil...

Страница 36: ...probe tip will increase the rate of future erosion For that reason it is recommended that after every 5 or 6 hours of use the tip be examined and if necessary polished with a fine emery cloth Since t...

Страница 37: ...ve step by step instructions to help remedy the problem If the Start button does not initiate sonication please go to the Option Screen Check the Footswitch option and even if you are not using a Foot...

Страница 38: ...visit our website at www sonicator com and fill out our brief Repair Form This form provides us with the necessary information to quickly diagnose your problem and will speed up the repair of your equ...

Страница 39: ...plant in Farmingdale New York or at any of its designated service centers without charge including parts and labor but not including shipping charges necessitated by any defect in workmanship or mater...

Страница 40: ...U2000A0CL4 both are needed The total cost for the boxes and foam is 50 00 including shipping to any US location QSonica is not responsible for shipping damage on equipment returned to our facility for...

Страница 41: ...sample If the water temperature rises pour it out and refill the cup with cold water Another method of operation is to fit the outlet port with the hose adapter soft tubing and a pinch clamp Run the...

Страница 42: ...rtical diaphanous streams in the center of the cup sometimes forming a spider like pattern Output Amplitude Settings When working with small samples there is a tendency to turn the Amplitude setting t...

Страница 43: ...43...

Страница 44: ...a clear acrylic collar to contain the liquid media within the reservoir This format allows the user to process deep well microplates or other large vessels Standard microtiter plates or pcr tubes requ...

Страница 45: ...at the bottom of the Acoustic Enclosure Connect the cable to the converter and slowly feed the cord down through the bottom of the enclosure until the converter sits properly in the holder be sure tha...

Страница 46: ...rough air it requires a liquid medium to be transmitted effectively If air pockets are present and water is not in contact with the bottom and side of each well sonication will be inefficient and your...

Страница 47: ...ts The optimum scenario is to re circulate ice cold water with pulsed sonication Maintenance To ensure optimum performance it is highly recommended to remove the horn from the converter and clean the...

Страница 48: ...Connector Microplate Horn Tygon Tubing Stud Pinch Clamps The tall clear collar is removable If it is not required simply slide it upwards and it will be released The Sound Enclosure is included with t...

Страница 49: ...Corning Costar 1 800 492 1110 Chillers Peristaltic Pumps Thermo Fisher Catalog Chillers Catalog 13 874 7 Model 3006 capacity 6L Catalog 22 037 157 Model WK 230 Benchtop chiller capacity 6L Thermo Fish...

Страница 50: ...her end of the body and the mating surface of the Horn and fit the body over the Horn 8 Screw the Flocell body around the Horn until the end of the horn thread contacts the orifice plate and then back...

Страница 51: ...body over the Horn 8 Screw the Flocell body around the Horn until the end of the horn thread contacts the orifice plate and then back it off 1 full turn 9 The Flocell should not be tightened on the h...

Страница 52: ...ure proper installation of the horn into the Focell body 7 Push horn into upper section of Flocell until fully seated 8 Place 3 sanitary gasket on upper section flange of Flocell body 9 Place each hal...

Страница 53: ...53...

Страница 54: ...be clean tight mating surfaces Please refer to Maintenance section of this manual for direction on how to clean the threaded mating surfaces and ensure that the threaded studs are attached tightly to...

Страница 55: ...extender horns are tested in pairs at the factory to ensure balanced operation and consistent output when used together It is highly recommended to replace both horns at the same time Mixing extender...

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