Photometrics Evolve 512, Руководство пользователя

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Evolve 512 Users Manual 23
Top-Lock
This advanced feature effectively functions as an intensity filter for the camera. It allows
users to select the background level at which the EMCCD will make all pixels at or above
the selected value equal to the value selected.
How will it help?
This advanced feature allows users to define an effective roof for an image at a level which
they select. In many experimental conditions, there may be intense objects in the field of
view that are actually of little or no interest to the experimenter. A simple example may be a
piece of debris in the field of view that happens to be highly fluorescently labeled. If the
debris is not of interest, a top intensity value of the image can be selected so that every
single pixel in the image at or above the selected top intensity value will be displayed as the
selected top value.
Another example where Top-Lock may be important is when researchers are looking for
small, low-intensity-labeled features that are actually budding off or being released from a
large feature. A specific example would be something like small vesicles being released from
the Golgi apparatus in a cell. If the researcher is only interested in observing and tracking
these features but the highly labeled Golgi is swamping the image display, then the camera
can be set to lock all intensities above a certain level to that level. This will make the features
the researcher is interested in much more visible, as well as scaled nicely within the display
image for ready visualization.
Combining Black-Lock and Top-Lock
Utilizing Top-Lock and Black-Lock together allows users to narrow visualization rapidly
and effectively into the intensity range of the image features they are most interested in.
Black-lock and Top-lock in action
For further explanation, including a real-time demonstration of black-lock and top-lock,
please visit
www.evolve-emccd.com
Dual-Readout
Port Operation
The Evolve 512 is configured with software-selectable dual-readout amplifiers, also referred
to as "ports", shown in the next figure. The two amplifiers are listed below.
Port #1: On-chip Multiplication amplifier
Port #2: Traditional or non-multiplication amplifier
Figure 19. Evolve 512 CCD Array Structure