PerkinElmer Migele, Руководство пользователя

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Troubleshooting
Problem Cause Action
Gel separated from backing during
blotting
Too much pressure applied to the
blotting paper
Use a light touch when blotting the
gel.
Blotting paper rested on the gel too
long
Blot gel for approximately 10
seconds.
Resulting pattern appeared skewed Wicks were not applied parallel to
each other
Place wicks exactly parallel to each
other on the gel; use the grid to help
in positioning the wicks.
Gel burned along one of the electrode
wicks during focusing
Wicks extended beyond edges of gel Level chamber to prevent water from
migrating to wick edge. Blot gel
during run by pausing power supply
and using paper towel to blot entire
gel.
Voltage decreased and current
increased during initial phase of a run
Anode wick was placed in a cathode
wick location
Consult the test instructions and
reposition wicks (within 10 minutes of
starting the run).
Leads connected to the power supply
were reversed
Connect red lead to red positive
terminal and the black lead to the
black negative terminal.
Burning along the edges of the gel Too much water beneath the gel Apply gel to cooling surface; blot the
gel periphery to remove excess water
beneath the gel surface.
Too much water beneath the gel Use minimum amount water under
gel and blot gel edge prior to starting
the run.
Run not started Power supply wiring disconnected Check the wiring and the connections.
Lid in "idle" position Press the lid into "use" position,
monitor with the green label at the
front panel of the instrument.
The tray is not closed Close the tray.
Focusing did not finish in the
prescribed time
Uneven contact between the
electrodes and the wicks
Electrodes must rest firmly along the
centre of the wicks.
Electrodes not clean Clean electrodes with paper and
distilled or deionized water.
Uneven migration of the bands Uneven contact between the
electrodes and the wicks or uneven
saturation of wicks with solution
Electrodes must rest firmly along the
centre of all electrode wicks and blot
wicks prior to application onto gel.
Insufficient cooling Monitor the circulating water bath
performance and use +10 - +15 °C as
lowest running temperature.
Samples run together Contaminated sample template Clean templates after each use
with distilled or deionized water, or
replace the templates.
Air bubbles trapped under the
template
Lightly run finger between the wells
of the template to remove any air
bubbles.
Gel not blotted sufficiently Use parabola technique when placing
template on the gel. Blot gel for
approximately 10 seconds before
placing it on the electrophoresis unit.
2118-0010 - Migele
®
Gel Electrophoresis Unit User Manual
Troubleshooting
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