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on page 24). After runs ensure that the tubing and wires are in good condition and not worn out (if the

instrument is old). If you have any questions, please contact your local PerkinElmer representative.

Figure 21: Lid in idle position

Figure 22: Lid in locked position

2118-0010 - Migele

®

Gel Electrophoresis Unit User Manual

Operation

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Содержание Migele

Страница 1: ...2118 0010 User Manual Migele Gel Electrophoresis Unit ...

Страница 2: ......

Страница 3: ...ystem 8 Cooling plate 8 Lid with electrodes 9 Installation 11 Connecting the cooling system single unit 13 Levelling the instrument 14 Setting the lid 17 Connecting the power supply 17 Connecting the cooling system multiple units 18 Stacking several GEUs 18 Operation 21 Cooler setting 21 Power setting 21 Electrode setting 21 Placing gel 22 Closing the unit 22 Starting the run 23 Ending the run 23 ...

Страница 4: ...Decontamination information 28 Technical specifications 29 Troubleshooting 30 Contact addresses 32 WEEE Instructions for PerkinElmer Products 33 Warranty 34 Index 35 ii ...

Страница 5: ...tories Intended purpose The Migele Gel Electrophoresis Unit is intended to be used together with the RESOLVE Hemoglobin Kit in screening neonatal hemoglobinopathies and as an aid in the diagnosis of adult hemobloginopathies It is used together with a power supply and a circulating water bath for qualitative separation of hemoglobin variants in whole blood cord blood or dried blood spot specimen ap...

Страница 6: ...or use In vitro diagnostic medical device Catalogue number Serial number This way up Fragile handle with care Keep dry Certification mark from testing laboratory Follow national guidelines when disposing of the equipment 2118 0010 Migele Gel Electrophoresis Unit User Manual General information 2 ...

Страница 7: ...ould cause damage to the instrument or loss of data or personal injury to the operator or the patient if correct procedures are not followed Note A note emphasizes significant information in a procedure or description and alerts users to key points of interest not related to personal injury In cases where a specific hazard or warning has been identified such as an electrical or burn hazard that sy...

Страница 8: ...e contact information for the manufacturer of this device to report a serious incident is Wallac Oy Mustionkatu 6 FI 20750 Turku Finland http www perkinelmer com Phone 358 2 2678 111 2118 0010 Migele Gel Electrophoresis Unit User Manual Safety information 4 ...

Страница 9: ...om Wallac Oy or PerkinElmer Inc and its distributors Programmable Power Supply CWP 2000 Circulating Water Bath 1023 0010 1023 0020 Resolve Hemoglobin Kit FR 9120 FR 9360 FR 9400 Resolve Hb FASC Control Kit 3412 0010 In addition the following installation tools are required but not provided with this package 16 mm wrench for attaching tubing to Circulating Water Bath screw driver slot head for atta...

Страница 10: ... mm FR 9120 Additional equipment required but not supplied with the instrument Programmable power supply CWP 2000 or equivalent Voltage at least 1500 V Power at least 80 W Current at least 200 mA Circulating water bath 1023 0010 1023 0020 or equivalent Temperature setting 10 15 C Flow rate at least 5 l min Maximum pressure below 0 5 bar Cooling power at least 160 W at 10 15 C Figure 1 Migele Gel E...

Страница 11: ...Electrophoresis Unit connections Water tubing is shown on the right and the power supply connections are shown on the left part of the instrument 2118 0010 Migele Gel Electrophoresis Unit User Manual Instrument overview 7 ...

Страница 12: ...e Gel Electrophoresis Unit system Cooling plate Ceramic aluminium oxide cooling plate measures 260 x 260 mm supports the gel and provides uniform temperature control Aluminium oxide is an excellent heat conductor and electrical insulating material To facilitate the correct positioning of electrophoresis gels the surface of the cooling plate is screened with a grid measuring 240 x 240 mm Figure 3 G...

Страница 13: ... accordance with the grid and gel requirements The instrument can be run with two or one anode electrode modes The lid contains three electrodes but one anode electrode can be disabled with the levers if not in use See the pictures below for more details Figure 4 Disabling anode electrode with the levers 2118 0010 Migele Gel Electrophoresis Unit User Manual Description of parts 9 ...

Страница 14: ...trode The distance from the cathode electrode can be varied from 6 cm to 10 cm One of the anode electrodes can be lifted to remove it from operation if the instrument requires to be operated in two electrode mode small gel e g FR 9120 kit The lid can be left in the idle position to protect the electrodes and ventilate the cooling plate 2118 0010 Migele Gel Electrophoresis Unit User Manual Descript...

Страница 15: ... 9400 FR 9360 RESOLVE Hemoglobin kit and the 3412 0010 Resolve Hb FASC Control kit or equivalent controls are needed not included Use the same kit and gel that you are planning to use for the samples As the Migele Gel Electrophoresis Unit is intended to control the gel processing condition avoid warm exhaust air from other instruments when placing the instrument Leave sufficient space behind the i...

Страница 16: ...Figure 7 Removing transport protection Figure 8 Removing protective plastic sheet 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 12 ...

Страница 17: ...e package contains tubing with the corresponding connectors to be used with the circulating water bath Install the connectorsto the circulating water bath using the clamp supplied with the water bath Ensure that direction of the flow is correct circulator out to unit in see Figure 10 Tubing for the water bath on page 14 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 13 ...

Страница 18: ...reservoir with cooling liquid water Caution Ensure that tubing is not bent too severely kinked This blocks the coolant flow and gel run may be affected Note Using HI setting in the circulator pump speed is recommended Levelling the instrument When the Migele Gel Electrophoresis Unit is in the final bench location place a spirit level on the cooling plate see Figure 11 Spirit level to monitor that ...

Страница 19: ...Figure 11 Spirit level to monitor that the instrument is levelled correctly 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 15 ...

Страница 20: ...n the locking nuts Adjust first the back feet to get the device on level sideways Then adjust front feet to get the device on level front to back Then adjust the other front feet to get the device to sit firmly on the table You can turn the device on the side to get better access to tighten the locking nuts 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 16 ...

Страница 21: ...gele Gel Electrophoresis Unit or ensure that they have a suitable DC insulation compliance for the used voltages Connect the supplied wires from the power supply to the back plate of the Migele Gel Electrophoresis Unit please use wires of corresponding colors One power supply can be used with two to four units in parallel depending on power supply type used See the pictures below for more details ...

Страница 22: ...t the units in series with the attached tubing Caution Ensure that tubing is not bent too severely kinked This blocks the coolant flow and gel run may be affected Stacking several GEUs Connect the GEUs together with the supplied clamp Connect the lowest unit also to the bench table Connect the GEUs in series with the attached tubing stacking Up to four units can be stacked Caution Ensure that tubi...

Страница 23: ...Figure 14 Connecting the units 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 19 ...

Страница 24: ...Figure 15 Stacked GEUs 2118 0010 Migele Gel Electrophoresis Unit User Manual Installation 20 ...

Страница 25: ...red temperature Power setting Set the desired run profile according to the kit insert Ensure that the power supply wires are connected before the power unit is turned on Electrode setting Adjust the cathode and anode spacing to the desired distance using the thumb screws loosen set location tighten see Figure 16 Black thumb screws for setting anode position on page 21 Figure 16 Black thumb screws ...

Страница 26: ...cooling plate is clean Follow the kit insert instructions when positioning the gel to the cooling plate You can use the grid to position the gel Cathode wick needs to be positioned in the 0 position Closing the unit Close the lid to the locked position indicator label in the front panel shows green see Figure 18 Locked position indicated by green spot visible on page 22 Push the tray to the inward...

Страница 27: ...h the power supply to pause if the excess moisture is condensed inside the instrument Blot any excess water that may appear on the gel during the electrophoresis with a blotting paper Ending the run Turn the power supply off Pull the tray to the outward position Remove the gel from the cooling plate and process it according to the kit insert instructions After each run the plate should be cleaned ...

Страница 28: ...in good condition and not worn out if the instrument is old If you have any questions please contact your local PerkinElmer representative Figure 21 Lid in idle position Figure 22 Lid in locked position 2118 0010 Migele Gel Electrophoresis Unit User Manual Operation 24 ...

Страница 29: ...onment and laboratory practice will not cause excess condensation Compare results with picture below Gel bands should be straight and positioned similarly over the cooling plate If there is anything that is not positioned correctly please take a picture of the gel and send it to your local PerkinElmer representative to be investigated If the water is not run enough through the cooling plate before...

Страница 30: ...the run and if required also before the run Solvents and detergents should not be used in cleaning the cooling plate surface See the pictures below for more details Figure 24 Cleaning the cooling plate and lid Lid When not used leave the lid in the idle position stand by to ventilate the cooling plate and electrodes This can be monitored at the front panel of the instrument with the green spot ind...

Страница 31: ...tions provided in Replacing the lid on page 27 Monthly maintenance We recommend you to make sure that the connections of the instrument tubing and wiring are in good condition and not worn out once every month Also check the electrodes If they are worn out contact your local PerkinElmer representative Replacing the lid The lid with three electrodes can be purchased as a spare part and assembled wi...

Страница 32: ...eionized water or with a 70 80 ethanol solution Do NOT use any other liquid for cleaning Decontamination information form Product type ________________ Serial no ________________ This is to inform those who maintain repair or transfer the product that the instrument and its subunits have been treated according to the procedure given above Date ________________ Signature ___________________________...

Страница 33: ...e parts are resistant to solvents used in the kits manufactured by Wallac Oy If other chemicals are used the customer is responsible for testing them Electrical safety requirements The instrument meets the following safety standards EN 61010 1 61010 2 101 Operating environment Temperature 18 30 C Relative humidity 20 80 relative humidity non condensing Storage temperature 0 40 C Normal laboratory ...

Страница 34: ... Use minimum amount water under gel and blot gel edge prior to starting the run Run not started Power supply wiring disconnected Check the wiring and the connections Lid in idle position Press the lid into use position monitor with the green label at the front panel of the instrument The tray is not closed Close the tray Focusing did not finish in the prescribed time Uneven contact between the ele...

Страница 35: ...volume of electrode solutions Use the recommended electrode solution at the specified concentration Excessive power setting Check the power setting Sparking along edge of gel onto cooling plate Excess moisture on gel or under cooling plate Remove the excess moisture Electrode strips overhanging the ends of the gel Cut the electrode strips short of the ends of the gel Liquid expelled at sides of el...

Страница 36: ... FIN 20101 Turku Finland Tel 358 2 2678111 Fax 358 2 2678 357 Email info perkinelmer com Website www perkinelmer com Service Please contact your local PerkinElmer representative 2118 0010 Migele Gel Electrophoresis Unit User Manual Contact addresses 32 ...

Страница 37: ...recovery programs vary by regulatory authority at your location Contact your local responsible body e g your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the website listed below for information specific to PerkinElmer products Website www perkinelmer com WEEE Europe follow the link provided above to access instruc...

Страница 38: ...trument is free from defects in materials and workmanship During the Instrument Warranty Period Wallac will provide parts and labor to repair defects in materials and workmanship Note The warranty on this instrument does not cover required Periodic Maintenance PM if any the cost and expense of which must be borne by the purchaser or renter Failure to perform required PM shall void the Warranty Per...

Страница 39: ...EU 13 Connecting power supply 17 Levelling instrument 14 Setting the lid 17 Stacking several GEUs 18 Instrument Migele GEU 6 Power Supply Circulating Water Bath 6 Intended purpose 1 IQ OQ 25 M Maintenance 26 Daily 26 Monthly 27 O Operation Closing unit 22 Cooler 21 Electrode 21 Ending run 23 Gel 22 Power 21 Starting run 23 P Parts Description 8 S Safety information 3 Specifications 29 W Warnings 3...

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Страница 42: ...2118 9010 03 April 28 2022 Manufacturer Wallac Oy Mustionkatu 6 FI 20750 Turku Finland Tel 358 2 2678 111 Fax 358 2 2678 357 www perkinelmer com ...

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