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a.
Y Unit
: Select one of the display unit : Absorbance, Transmittance, Reflectance or Energy.
b.
SBW (nm):
Select bandwidth. There are five bandwidths selectable: 0.5, 1, 2, 5 or 20 nm.
c.
Tm Method
: Select a method for determining melting temperature. Options include: 1st
derivative and Average.
d.
Equation Name:
Enter the name of the formula. %G-C is entered as default, of which
equation is formulated in
Equation Expression
.
e.
Equation Expression:
The default equation for the calculation of %G-C base pairs is:
%G-C=2.44 * (Tm - 81.5 - 16.66 * log(M) + 500/K)
Where M is the molarities of salt in mol/L, K is the DNA base pair length. %G-C will be
calculated with estimated T
m
and input values, Molarities (M) and length of DNA (K).
f.
DNA Pair Length(K)
Enter the DNA base pair length. This value will be used calculating %G-C.
g.
Calculation Start Temperature (°C):
Enter the start temperature for calculating the T
m
value.
h.
Calculation End Temperature (°C):
Enter the end temperature for calculating the T
m
value.
i.
Molarity (mol/L):
Enter the salt molarities of the solution [mol/L]. This value will be used in
calculating %G-C.
7.
After setting parameters for
Experiment
and
Lamp
, click the
Accessory
tab. And select
Multi-
Cell Peltier
.
Содержание Lambda 365 Peltier Temp Ctrl Unit Multi
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