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Instruction Manual peqPOWER 300 Volt Power Supply
OPERATIONAL ELECTRICAL PARAMETERS
Power Considerations
Electrophoresis is the migration of a charged particle under the influence of an electrical field. The
power supply output parameters voltage, current and power are related by the following two equations:
Voltage (V) = Current (I) x Resistance (R); (V=I*R)
Power (P) = Current (I) x Voltage (V); (P=I*V)
Resistance
Resistance of the assembled electrophoresis cell is dependent on the conductivity of the gel buffer, the
thickness of the gel, and the number of gels being run. Although the resistance is determined by the gel
system, the resistance can vary over the course of an electrophoretic separation.
Voltage
The velocity with which an ion moves in an electric field will vary in proportion to the field strength (volts
per unit distance). The higher the voltage the faster an ion will move.
Current
Current is a function of the number of ions passing a given cross-section of the circuit at a given time.
For a given gel/buffer system, at a given temperature, current will vary in proportion to the field
strength (voltage) and/or cross-sectional area (number and/or thickness of the gels).
Power
The power in Watts, or the rate of heat generated by the system, is directly proportional to voltage and
current (P=I*V).
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