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Gas connections
Air, carbon dioxide, oxygen and nitrogen are connected to the
iCELLis 500+ control system using Swagelock ¼ in. tube OD (female
connector on the iCELLis 500+ control system).
All gases must be supplied dry and oil free and they must be regulated to a
maximum of 2.5 barg (36.3 psig).
Connections provided for
additional input/output
1 spare connector available at the back of the machine for additional
input/output based on customization request.
Sensors
Single-use pH sensors
2 optical based disposable pH sensors for measurement of disposable
bioreactor internal pH, 1 sensor at the center before the fixed-bed, 1 sensor
after the fixed-bed.
Single-use DO sensors
2 optical based disposable DO sensors, 1 sensor at the center before the
fixed-bed, 1 sensor after the fixed-bed.
Single-use pressure sensors
1 disposable pressure sensor for measurement of disposable bioreactor
internal pressure.
1 disposable pressure sensor for measurement of double jacket line
pressure.
Temperature sensors
2 pt100 sensors for measurement of disposable bioreactor internal
temperature.
Functions
Agitation
Agitation is provided by a bottom-mounted impeller in the iCELLis
disposable bioreactor. The impeller is magnetically driven. The impeller
speed can be set via the iCELLis 500+ software between 0 and 450 rpm.
Temperature control
Bioreactor internal temperature control using double jacket integrated in the
disposable bioreactor and external TCU.
Aeration
Gas overlay: Air, carbon dioxide, oxygen and nitrogen, can be delivered at
a maximum combined gas flow rate of 7 NL/min.
Gas MFC
Max. Flow
Capacity (L/min)
Automatic
Mode (L/min)
Manual
Mode
(L/min)
CO
2
1.5
1.5
1.5
N
2
1.5
1.5
1.5
Air
3
3
3
O
2
7
7
7
Total Gas Flow
13
0 to 7
13*
*It is only possible to achieve the maximum total gas flow capacity in the
manual mode of each line, setting each line to its maximum value. The total
gas flow will be the sum of all the maximum individuals gas flows (13L/min)
Pumps
Variable speed peristaltic pumps from 0 to 360 rpm (minimum
recommended speed is 25 rpm).
Maximum theoretical flow of the Watson Marlow
♦
520 pump head with high
flow manifold (PharMed BPT ID:
3
/
8
in.): 5,4L/min
Maximum theoretical flow of the Watson Marlow 520 pump head with low
flow manifold (PharMed BPT ID: ½ in. ): 2,4L/min
Weight measurement
Load cells provide ability to measure weight of the iCELLis 500+ bioreactor
Bioreactor over-pressure
protection
Interlock using disposable pressure sensor for measurement of bioreactor
internal pressure
