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the focus down to visualize the tip in the Video panel. As focus changes, the user may adjust illumination
intensity in the video panel (Fig 9b)
; using the mouse, left click on the “I” slider at the bottom of the
video panel and drag to the left or right. Also, the user may zoom in/out of the view using the slider on
the lower left-hand corner of the Video panel.
In the Video panel (Fig 9b), use the mouse pointer to indicate a location on the tip. At this location, right
click to
“spot on” the laser location
. This is the detector laser. This laser spot should be close to the tip,
without spilling over the edges of the cantilever. Adjusting the light intensity will help to view this. To
make very fine adjustments to the laser location, use the buttons in the crosshair on the top left-hand
corner of this panel; holding the Shift key+clicking a button moves the laser in the smallest possible
increment. When the laser location is satisfactory, zero the detector by clicking the red dot in the center
of the crosshair on the left-hand side of the Engage Panel. You should see the Deflection value in the
Sum and Deflection panel decrease to 0-0.10 (Fig 10)
In the Video panel (Fig 9b), use the mouse pointer to indicate a location very close to the base of the
cantilever, and right click in this location to
“spot on” the BlueDrive laser
. This laser is not blue; it is red
and appears larger than the detector laser. Small adjustments to the BlueDrive laser location can be
made with the crosshair on the top right-hand corner of the Video panel.
In the Video panel (Fig 9b), use the mouse pointer to indicate a location very, very close to the tip and
right click to select
“
tip position
”
.
This action marks the location of the tip in the Video panel with a
green crosshair. The green crosshair remains in the video panel to indicate to the user where the tip will
land on a sample surface. In the Engage panel,
find the “Focus on Tip” button and
click the
“SET”
button
to the immediate right. This completes all the steps for locating and marking tip locations.