OWL The Otter SGC-1, Руководство пользователя

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U
sing the System
SECTION 2
8
5. Slowly pour gel solution onto the
bottom plate at the point where the
two plates overlap. The acrylamide
will flow into the space between
the two plates by capillary action.
Gently slide the top plate across the
bottom plate while pouring gel solu-
tion along the leading edge of the
glass. If a bubble forms, simply
move the top plate backwards until
you have passed the point where the
bubble formed and then proceed for-
ward again until the gel is completly
cast.
6. Clamp along the spacers in the
spaces provided by the caster. Clamp
in the middle of the spacer and not in
the gel area.
7. Insert comb (see page 9 for
Sharktooth and Well Comb instru-
tions) at the top of the gel and clamp
with two or three clamps as space
allows. Clamp in the middle of the
comb and not on the teeth or beyond
the comb into the gel area.
8. After gel has polymerized for
20-30 minutes, the cassette can be
removed from the caster and allowed
to complete polymerization on the
benchtop. Do not move the gel dur-
ing the first minutes of polymeriza-
tion.