OWL The Otter SGC-1, Руководство пользователя

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echnical Tips
T
SECTION 3
10
The difference between notched and offset glass:
Notched or eared glass plates face in towards the
upper buffer chamber during a gel run. The “ears”
are glass tabs on each side of the plate, which
prevent buffer from running out of the upper buffer
chamber.
Offset glass plates serve the same purpose as
notched plates. Offset glass is usually about 2 cm
shorter than the front, or blank, glass plate. Instead
of glass ears, pieces of adhesive sponge are adhered
either to the top of the spacers or to the apparatus
itself. These sponge tips wear out and may leak.
Notched glass plates are more fragile and expen-
sive than offset glass and sponge tips, but many
researchers prefer notched glass because it is easier
to use.
The third spacer:
This is a bottom spacer, and can be cut to the
desired length. It is optional. If you use a bottom
spacer when casting, remember to remove it before running the gel.