Moxi Flow
™
User Guide
Page 26
Troubleshooting
Symptom
Cause
Corrective Action
Battery will not fully charge
Battery is faulty or has surpassed
its service life
Return instrument for battery replacement
Fluid not pulling into cassette
Test type not selected
Select the assay/test to run on the
Home
screen
Doors not closed
Close doors and select a test type
Cassette manufacturing error
Try other side of cassette or a new cassette.
Pump error
If the above steps don’t resolve the issue, it is
possible there is an error with the instrument
pump. Contact Orflo Technical Support.
Instrument stops responding
Internal firmware issue due to
instrument malfunction or high
level of external interference
Reset instrument by pressing and holding the
power button for at least 5 seconds. If problem
persists, return instrument for service.
No Clear Scatter (Dot) Plot
clusters or “smearing” of scatter
plot output
Cell/Bead concentration too low
Try running the cell sample at a higher
concentration.
Sample Flow Issue
This is a situation that can potentially occur when
running over-concentration, highly-aggregated,
and/or "sticky" samples that result in clogging of
the filters and/or aperture. This can result in
stopped or hindered flow that affects the
fluorescent and impedance signal. Dilute the
sample further and try to further dissociate the
sample with pipette trituration and/or protease
(e.g. Accutase) treatment
Low/protein expression
For poorly expressed proteins, surface labeling of
the protein might not provide a sufficient
fluorescent separation from the dark sample.
No Fluorophore present
Cell preparation protocol labeling step was
omitted.
Questionable concentration
Concentration of cell/bead sample
is too high or too low
Make sure concentration of cell or bead sample is
within recommended guidelines. Refer to
Specifications section.
Wrong diluent
Cells should be suspended in 0.9% salt solution
(e.g. PBS or equivalent) to ensure proper
conductivity for unit operation and to ensure
proper particle sizing. Water, hypotonic, or
hypertonic solutions are
not
acceptable diluents.
Cell clumping
Ensure the cells are in a single-cell suspension.
Break clumps by pipetting up and down with a
standard pipette. Protease treatment can also be
used to dissociate clusters. ORFLO recommends
Accutase or equivalent.
Improper Size Gating
Please refer to the “Managing the Data” | “Gating
the Data” section for instructions on gating. The
noise/debris cluster in the bottom left corner
should be excluded from count analysis.
Questionable cell diameter
Wrong diluent
Cells should be suspended in 0.9% salt solution
(e.g. PBS or equivalent) to ensure proper
conductivity for unit operation and to ensure
proper particle sizing. Water, hypotonic, or
hypertonic solutions are
not
acceptable diluents.
