Orflo Moxi Flow Скачать руководство пользователя страница 3

Страница: 3 / 39

Contents

Introduction ................................................................................................................................... 1

About the User Guide ................................................................................................................... 1

Conventions Used in the User Guide ........................................................................................... 1

Safety Precautions ........................................................................................................................ 2

General Safety .......................................................................................................................... 2

Laser Safety .............................................................................................................................. 2

Biological Safety ........................................................................................................................ 3

Moxi Flow

System Overview ..................................................................................................... 4

Moxi Flow

Accessories .............................................................................................................. 5

Getting Started .............................................................................................................................. 6

General Guidelines ....................................................................................................................... 6

Materials Required ........................................................................................................................ 7

Using the Moxi Flow

.................................................................................................................. 7

Home (Start) Screen ................................................................................................................. 7

Settings ..................................................................................................................................... 8

Passcode Lock ...................................................................................................................... 8

Fluorescence Gain ................................................................................................................ 9

Analyzing a Sample .................................................................................................................. 9

Sample Preparation Considerations ...................................................................................... 9

Running a Test ...................................................................................................................... 9

Test Screen Output ................................................................................................................. 12

Scatter/Dot Plot Output ........................................................................................................ 12

Histogram Output ................................................................................................................ 13

Managing the Data .................................................................................................................. 14

General Recommended Post-Test Workflow ...................................................................... 14

Re-scaling the x axis (size range) ....................................................................................... 15

Removing the noise region .................................................................................................. 15

Gating the data .................................................................................................................... 16

Содержание Moxi Flow

Страница 1: ...For Research Use Only Moxi Flow is not for use in diagnostic procedures MOXI FlowTM USER GUIDE...

Страница 2: ...is manual 2013 ORFLO Technologies a DBA of E I Spectra LLC All rights reserved Rev 1 4 3 5 2016 The ORFLO symbol and Moxi Flow are trademarks of E I Spectra LLC registered in the U S and other countri...

Страница 3: ...d 6 General Guidelines 6 Materials Required 7 Using the Moxi FlowTM 7 Home Start Screen 7 Settings 8 Passcode Lock 8 Fluorescence Gain 9 Analyzing a Sample 9 Sample Preparation Considerations 9 Runnin...

Страница 4: ...ata Connecting Via USB USB on the go 21 Instrument Firmware and Software Upgrades 24 Updating Moxi FlowTM Firmware via USB 24 Moxi FlowTM Apps 25 Troubleshooting 26 Maintenance and Storage 29 Ordering...

Страница 5: ...can store up to 16Mb of data and if desired the data may be downloaded to a PC or Mac via USB connectivity The system is intended for research use only and has been tested with cell types that are rep...

Страница 6: ...t attempt to disassemble or service the Moxi FlowTM The instrument has no user serviceable parts All service must be performed by ORFLO Technologies WARNING To avoid the danger of fire or explosion do...

Страница 7: ...ollowed to minimize the risk of exposure to biohazardous materials Handle all biological samples as if they are capable of transmitting harmful infections Wear appropriate personal protective equipmen...

Страница 8: ...TM Kit includes the Moxi FlowTM instrument USB Cable Power Adapter US UK and EU versions only User Guide and one pack of 25 Cassettes USB Cable Port Touch Screen Display Power Reset Button Lower Door...

Страница 9: ...5 seconds Lower Door Manually opened and closed by user to allow insertion of a test cassette Upper Door Manually opened and closed by user to allow loading of a test sample Door must be closed prior...

Страница 10: ...to the instrument NOTE Tests should only be performed with the instrument powered by an AC source Although the instrument has an internal rechargeable battery Orflo strongly recommends that tests not...

Страница 11: ...e Open Flow Cytometry Orflo approved kits reagents are strongly recommended Materials Required Cell or bead sample diluted and dissociated if necessary 75 L minimum MF S or MF M cassette Pipette and a...

Страница 12: ...e Settings screen to highlight the Passcode Lock field Use the up or down arrows to change the Passcode Lock field value to On and select Done The system will present a keyboard to enter the four digi...

Страница 13: ...For Type MF M cassettes a dilution of 1 5 to 1 20 is recommended for most mammalian cell lines but the appropriate dilution will depend on cell type and seeding density Type MF S cassettes will typica...

Страница 14: ...the gray box red arrow in image to change this gain Four options in order of increasing gain for the fluorescence signal output are possible Low Default Medium High The initial value populated in thi...

Страница 15: ...ing for the fluid to reach the start electrode If the time limit for any of the steps is exceeded the test will automatically cancel and the user will be returned to the Home screen 6 The Moxi FlowTM...

Страница 16: ...est output screen components are fully labeled in the images below for both the scatter dot plot output and the size histogram output More detailed workflow instructions for each function are outlined...

Страница 17: ...Moxi Flow User Guide Page 13 Histogram Output...

Страница 18: ...ate time Note the disk usage percentage is indicated in the upper right corner of this screen Selecting a thumbnail opens that test for editing identical to the post test editing with the exception th...

Страница 19: ...that resolution is not lost during the rescale process 1000 histogram bins This allows and is recommended for users to achieve finer resolution of smaller particle populations and potentially increase...

Страница 20: ...r can even be dragged around the left edge of the scatter plot if needed right image below Events to the left of the left size gate and to the right of the right size gate are not included in the coun...

Страница 21: ...low also allows the user to store up to five gate locations in memory for recall A given set of gate settings can be saved by hitting the STO button Doing so brings up a list of five memory locations...

Страница 22: ...in order to properly view and gate the population As the lower display scale 2 m for MF S and 3 m for MF M is lower than the lower size detection limit of the system cassettes some inherent system noi...

Страница 23: ...ll counts and the green histogram are the live cell counts Pressing the Dot Plot button returns the screen to the initial scatter dot plot data Count Size and Percentage Information The Moxi Flow prov...

Страница 24: ...e Histogram tests an MPI value is also provided in the yellow box at the top right of the display This value is a measure of the purity of the sample by providing a ratio of the Curve Fit or Gated pop...

Страница 25: ...s can be generated from the Moxi Flow by selecting File Print Export Screenshot see above first image from the test screen Selecting this option will save a bitmap file named after the test with a bmp...

Страница 26: ...xi FlowTM will display a notification on the Home screen that it is Exporting files to disk The Moxi FlowTM disk will be mounted to appear as a drive on the computer following completion of this file...

Страница 27: ...the cassette 3 Press the Next icon to proceed to the next instruction screen or press the Previous icon to return to the previous screen The last screen of the help menu provides information on the v...

Страница 28: ...low into the computer that has the downloaded firmware update files 4 Next the user needs to put the Moxi FlowTM into a firmware update mode This can be done in either of the following two ways a With...

Страница 29: ...updated and is now ready for use Moxi FlowTM Apps Moxi FlowTM applications can be entered from touching the Apps icon on the Home screen Included applications in version 2 04 are Clock Programmable t...

Страница 30: ...e sample further and try to further dissociate the sample with pipette trituration and or protease e g Accutase treatment Low protein expression For poorly expressed proteins surface labeling of the p...

Страница 31: ...w the specified range of the cassette Re scaling the x axis can only be done after a test prior to saving can increase the binning resolution around these populations helping to better view the peak D...

Страница 32: ...oo small Make sure sample is loaded and volume is at least 75 L Test Error Incomplete Test Insufficient fluid 75 L Adjust pipette to ensure sufficient volume for the test For improperly calibrated pip...

Страница 33: ...ng time will vary but a complete charge is typically achieved in 4 8 hours The charge indicator in the upper right hand corner of the display see image to the right will change from flashing red to so...

Страница 34: ...m See the Technical Assistance section for information about contacting Orflo Product Description Cat No Moxi FlowTM Kit US Version MXF002 Moxi FlowTM Kit EU Version MXF003 Moxi FlowTM Kit UK Version...

Страница 35: ...s Open Flow Cytometry Assay requires R PE reporter Cassette Performance Type MF S Type MF M Preferred Sample Type Beads and Cells Larger Cells Diameter Size Range 3 16 m 4 22 m Cell Volume Range 14 21...

Страница 36: ...20 80 non condensing Altitude 2000 m maximum Applicable Pollution Degree Pollution Degree 2 Degree of Ingress Protection IPX0 Technical Service For technical service contact ORFLO Technologies at 855...

Страница 37: ...e or otherwise add to the coverage described here In accordance with EU requirements our standard product warranties remain in effect for two 2 years in EU countries What is Not Covered by Your Warran...

Страница 38: ...fitness for a particular purpose The warranty provided herein and the data specifications and descriptions of ORFLO products appearing in ORFLO s product literature may not be altered except by expre...

Страница 39: ...apply to the Moxi Flow and cassettes This is not intended to be an all encompassing list of related patents U S Patent 7 515 268 U S Patent 7 520 164 U S Patent 8 082 603 U S Patent 8 171 778 U S Pate...

Результаты поиска

Содержание Moxi Flow

Отзывы:

Бренды по названию

Популярные бренды

Orflo Moxi Flow, Руководство пользователя

Результаты поиска

Содержание Moxi Flow

Отзывы:

Бренды по названию

Популярные бренды