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DARKFIELD PROBLEMS 

Problem

 

Cause

 

Solution

 

It’s totally dark in the 
viewing field 

The light is not on 

Turn on the light 

(A191BOIL, A191BD only)

 There 

is no oil in between the condenser 
top lens and slide, when using 
100X objective 

Place a drop of oil on the top lens of 
condenser and let it contact the underside of 
slide 

(A191BOIL only)

 The built-in iris 

diaphragm of 100X objective is 
not in proper position 

Adjust the iris diaphragm ring on the 
objective 

The condenser is not in the right 
position 

Lower or raise the condenser slightly to the 
position that the specimen is the brightest. 

 

 

(A191BOIL, A191BD only)

 Make sure the 

oil contact the bottom of the slide and the top 
of the condenser during the adjustment. 

The illumination is 
insufficient or too bright 

The intensity of lamp is too low 

Adjust the intensity dial to increase the 
brightness 

The condenser is not in the right 
position 

Lower or raise the condenser slightly to the 
position that the specimen is the brightest.   
 

(A191BOIL, A191BD only)

 Make sure the oil 

contact the bottom of the slide and the top of 
the condenser during the adjustment. 

The condenser is not centered 
properly 

Adjust the translational centering screws to 
center the condenser 

(A191BOIL only)

 The built-in iris 

diaphragm of 100X objective is 
not in proper position 

Turn the iris diaphragm ring on the objective 

Image of the specimen 
is not clear and lacking 
in sufficient contrast 

The specimen is not suitable for 
darkfield observation 

Change to brightfield or phase contrast 

(A191BOIL only)

 The built-in iris 

diaphragm of 100X objective is 
not in proper position 

Turn the iris diaphragm ring on the objective 

The condenser is not in the right 
position 

Lower or raise the condenser slightly to the 
position that the specimen is the brightest.   
 

(A191BOIL, A191BD only)

 Make sure the oil 

contact the bottom of the slide and the top of 
the condenser during the adjustment. 

 

 

Содержание M827S Series

Страница 1: ...User Manual Binocular Biological Compound Microscope Model M827S Series MicroscopeNet com...

Страница 2: ...1 Component Illustration 3 2 Installation 4 3 Operation 7 4 Specifications 10 5 Optional Parts 11 6 Troubleshooting Guide 13 7 Darkfield Condenser Installation and Operation Instructions 15 8 Phase C...

Страница 3: ...icroscope is located on a smooth level and firm surface 4 If any specimen solutions or other liquids splash onto the stage objective or any other component disconnect the power cord immediately and wi...

Страница 4: ...ng an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning more difficult Oil immersion objectives...

Страница 5: ...Screw 19 Condenser Lock Thumb Knob 4 Nosepiece 12 Microscope Body 20 Condenser Focus Knob 5 Objective 13 Focus Tension Ring 21 Focus Stop Lever 6 Slide Holder 14 Coarse Focus Knob 22 Abbe Condenser 7...

Страница 6: ...mechanical stage is at its lowest position 2 Turn the caps counter clockwise to remove them from the nosepiece 3 Take the objectives out from the plastic cases and turn each one clock wise into the h...

Страница 7: ...t You may also loosen the two screws on the cover to adjust the position of the bulb to get centered and even brightness See Fig 3 7 Screw the cover on Caution Before you turn over the microscope be s...

Страница 8: ...no power is available 2 8 Installation of the camera optional may not included in your package 1 Take off the eyepiece from the eyepiece tube 2 Insert the camera into the eyepiece tube and then connec...

Страница 9: ...pupillary distance While observing with both eyes hold the left and right eyepiece tubes then slide the tubes in and out The interpupillary distance is correct when the left and right fields of view c...

Страница 10: ...ctive nosepiece to seat the 100X objective to the observing position until you hear a click sound 4 After observing the specimen use the lens cleaning paper to clean the 100X objective lens gently and...

Страница 11: ...ocus tension has been pre set at the factory If the mechanical stage drops by itself rotate the tension adjustment ring Fig 11 situated between the coarse focus knob and microscope body on the power s...

Страница 12: ...adjustment Focus Mechanism Coaxial coarse and fine focusing knobs on both sides w focus stop Minimum fine focusing adjustment at 0 002mm range 28mm Mechanical Stage Double layer dimension 5 1 2 x 5 1...

Страница 13: ...00X 1 25 oil 0 5 160 0 17 spring 37mm A191BOIL Oil 1 36 1 25 Plan 100X 1 25 oil 0 5 160 0 17 spring w iris diaphragm 37mm 2 Cameras Model Resolution Operating System Software A1502 640 x 480 0 3MP MS...

Страница 14: ...ring Achromatic 100X with built in phase plate spring oil five positions 10 for 10X phase contrast objective 20 for 20X phase contrast objective 40 for 40X phase contrast objective 100 for 100X phase...

Страница 15: ...too thick or thin Use a slide cover of the appropriate thickness 0 17mm Slide may be upside down specimen at the bottom Turn slide over so the cover glass faces up Diopter adjustment is not set proper...

Страница 16: ...brightness The condenser is not in the right position Lower or raise the condenser slightly to the position that the specimen is the brightest A191BOIL A191BD only Make sure the oil contact the bottom...

Страница 17: ...ondenser up or down slightly to get the best darkfield viewing Note The dry darkfield condenser is used with the dry objectives only The dry darkfield condenser works with the 4X 10X 40X objectives Th...

Страница 18: ...jective you will need to drop the immersion oil and adjust the iris ring Fig 16 to get proper brightness and contrast of view field 8 After observing the specimen with 100X objective use the lens clea...

Страница 19: ...ighten the thumb screw g Insert the light collector back onto the base and turn until it s tightened h Re adjust the condenser height to its normal position i Re adjust the mechanical stage height to...

Страница 20: ...e condenser control knob to raise the condenser to the highest position e Loosen the condenser lock thumb screw Fig 20 pull down the condenser and take it off f Insert the condenser annular ring disk...

Страница 21: ...onto the condenser as shown in Fig 22 c Loosen the condenser lock thumb screw as shown in Fig 23 take off the original condenser from the holder d Insert the phase contrast condenser into the condense...

Страница 22: ...g is in focus and the dark ring is visible i If the two ring images are not coincided as shown in Fig 24 c adjust the two centering screws on the condenser ring plate till the two rings are coincided...

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