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3) When using the 40X objective to observe the specimen
Place the slide on the stage.
Lower the condenser until a sharp brightfield image showing in the viewing field.
Raise the condenser until the darkfield image showing in the viewing field.
4) When using the 100X objective to observe the specimen
Raise the condenser until the top is close to the opening of stage.
Place a drop of immersion oil on the top of condenser.
Place the slide on the stage.
5) Raise the condenser and let the oil drop contact the bottom of the slide. If air
bubbles exist in the oil, clean the oil from the top of the condenser and bottom of
slide with a lens cleaning paper and repeat the procedures.
6) Follow the procedures in this manual to bring the sample on slide in focus and
observe.
7) (
A191BOIL Only
) When using the 100X oil darkfield objective,
you will need to drop the immersion oil and adjust the iris ring
(
Fig. 16
) to get proper brightness and contrast of view field.
8) After observing the specimen with 100X objective, use the lens
cleaning paper to gently clean the 100X objective lens, oil
darkfield condenser and the specimen in time.
9) If it is hard to clean, you need a little bit alcohol to clean the 100X
objective lens, oil darkfield condenser and the specimen.
Note
：
When using the 40X objective, you won
’t need to apply oil drop on the oil
condenser.
When using the 100X objective, the condenser won
’t work well if no oil drop
applied on the condenser.
When you apply the immersion oil with the 100X objective, do not let the
immersion oil to contact with the dry objective lenses (especially the 40X). If the
immersion oil is on the dry objectives lens, please use the lens cleaning paper to
clean the objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective
lens first.
Iris
Ring
Fig.16
