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(a) (b)
(c)
(d)
8
Phase Contrast Kit Installation and Operation
Instructions
8.1 Phase contrast kits of A1PHB1 or A1PHB3:
1) Mounting the phase contrast objectives
a. Take off all the objectives from the nosepiece.
b. Install the phase contrast objectives onto the nosepiece following the steps in
2.3
.
2) Mounting the phase contrast condenser/annular ring disk
a. Rotate the nosepiece and set the 4X objective in position.
b. Turn the coarse focus knob to raise the mechanical stage to the highest
position without contacting the 4X objective.
c. Turn the condenser control knob to raise the condenser to the highest position.
d. Loosen the light collector and take it off.
e. Loosen the condenser lock thumb screw,
pull down the condenser and take it off.
f. Insert the condenser/annular ring disk into
the condenser holder, and tighten the thumb
screw.
g. Insert the light collector back onto the base
and turn until it’s tightened.
h. Re-adjust the condenser height to its normal
position.
i. Re-adjust the mechanical stage height to its
normal position.
Note:
When raising the mechanical stage, do not
make contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 20X
phase contrast objective, you should turn the disk
at 20 as shown in
Fig. 17
.
5) Centering the annular ring.
a. Remove one eyepiece from the microscope
eyepiece tube and insert the centering
telescope as shown in
Fig. 18
.
b. Observe from the telescope. The bright ring
and dark ring should be coincided with each
other as shown in
Fig.19 (d)
.
c. If the ring images are not clear, turn the top of
telescope until both ring images are in focus.
d. If the bright ring is still obscure as in
Fig.19 (b)
,
adjust the condenser focus knob.
e. If the two ring images are not coincided as
Fig.19
Fig.18
20
Fig.17
