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OM0284

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LabGard® ES Energy Saver

Class II Laminar Flow

Biosafety Cabinet

Models

NU-543-300E/400E/500E/600E

Bench/Console

Operation and Maintenance Manual

July, 2021

Revision2

NU-543-300E Series E

NU-543-400E/500E/600E Series D

Manufactured By:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: (763)-553-0459

Содержание LabGard NU-543-300E

Страница 1: ...odels NU 543 300E 400E 500E 600E Bench Console Operation and Maintenance Manual July 2021 Revision2 NU 543 300E Series E NU 543 400E 500E 600E Series D Manufactured By NuAire Inc 2100 Fernbrook Lane P...

Страница 2: ...m particulate contaminants as prescribed in EN 12469 and NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other r...

Страница 3: ...ings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to...

Страница 4: ...stallation Assessments 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement 7 11 Digital Airflow Sensor Description and Replacement Section...

Страница 5: ...irflow Schematic and the application of environmental controls as required in the field of biological research or chemical containment The LFBSC when used with proper technique is an effective primary...

Страница 6: ...while specific agents are used o Which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operatin...

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Страница 8: ...OM0284 Page 8 of 72 Rev 2 July 2021 2 0 Models and Features The model NU 543E LabGard ES Class II Laminar Flow Biosafety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m...

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Страница 13: ...rning any item for any reason contact NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned to you as refus...

Страница 14: ...tion panel should be used A MINIMUM CLEARANCE OF 6 152MM IS REQUIRED FROM THE TOP OF THE CABINET TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX If this cabinet is used in a pharmacy appli...

Страница 15: ...ide a 2 51mm overhang and seal the interface of the bench and cabinet using RTV caulk as above 5 2 4 Gas Service NuAire doesn t recommend the use of natural gas within the LFBSC but if gas service is...

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Страница 17: ...ies Biosafety Cabinets may be hardwired optional or plugged into an outlet with protective earthing connection with the standard power cord The cabinet requires 230 VAC 50 60 Hz single phase current r...

Страница 18: ...city test Inflow velocity test Airflow smoke patterns Site installation assessment tests The testing methods and equipment required are specified on the factory inspection report included with this ma...

Страница 19: ...3 ea Sidewall One Backwall Left or Right Work Surface Cabinet Size Inches mm Height Fully Assembled Height Minimum for Transport Width Depth with Armrest Removed 60 7 8 1546 59 1499 41 5 8 1057 31 7...

Страница 20: ...te DC Motor Controller that provides automatic compensation constant volume control for both filter loading and line voltage variances Monitor and display airflow velocity performance via Intelliflow...

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Страница 23: ...user interaction to silence a window high audible alarm for cleaning loading purposes The alarm silence may last up to 15 minutes if the alarm condition still exists the audible alarm will again sound...

Страница 24: ...s may begin If the sliding window is raised an audible and visual alarm will occur but may be silenced by pressing the alarm silence key Airflow readings will not be displayed during the warm up perio...

Страница 25: ...larm description Audible alarms will produce an alarm tone for 30 seconds then ring back for 2 seconds of every 5 seconds Pressing the alarm silence key will silence the audible alarm for 15 minutes i...

Страница 26: ...hange Press key to back up one level or repeated times to return to normal run or standby mode 6 1 8 2 Time Menu The time menu contains both time of day and auto timer duration Upon selecting the time...

Страница 27: ...yed is provided by the airflow sensor in the cabinet s exhaust airstream The temperature displayed may indicate a slightly higher value than the laboratory room temperature due to some heat generation...

Страница 28: ...g should be evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the w...

Страница 29: ...precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Страница 30: ...lls Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and t...

Страница 31: ...re feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments Forearm elbow support The cabinet is provided with a non metallic fo...

Страница 32: ...ardous Drugs2 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal blower on b Open the hinged or sliding view screen and secure in the full open position With the vi...

Страница 33: ...disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protection Equipment Required Decontamination using hazardous gas vapor or mist must be performed in...

Страница 34: ...control center Reconnect power to the cabinet The outlets and blower can then be activated 9 Seal front and top openings using plastic and tape BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORA...

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Страница 36: ...dges of the front window sealed with a low residue tape 6 Tubing connected to the inlet and outlet of X10 7 Insertion of Hydrogen Peroxide H2O2 sterilant cup and desiccant cartridge to X10 8 Current B...

Страница 37: ...tamination system and cabinet Total duration is the total automated decontamination cycle time so both the cabinet Auto Decontamination time duration and the automated decontamination system cycle tim...

Страница 38: ...ssary to remove the lamp assembly 1 Switch cabinet light switch off 2 Remove the screws at each upper side of the control center and allow the control center to rotate down resting on the safety strap...

Страница 39: ...lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest wi...

Страница 40: ...76mm 20 508mm x 14 366mm x 11 1 2 292mm Filter Manufacturer Camfil Farr Camfil Farr NU 543 400E NuAire Part Number A 980962 02 A 980962 13 Filter Size 21 533mm x 44 1118mm x 3 76mm 20 508mm x 22 559m...

Страница 41: ...top of the hood Tighten the spring loaded bolts 4 places depressing the gasket material by 1 8 inch 3mm Step 5 Motor Blower Assembly Removal a It is recommended that the motor blower to be removed as...

Страница 42: ...ccess Calibration Service parameter To enter the service parameter menu perform the following Press and hold key then press ENT and keys sequentially releasing the key after the 3 key sequence The fol...

Страница 43: ...tor rpm is entered after the first airflow calibration is performed with clean filters The maximum rpm is a known fixed value based on the DC ECM motor used The difference between the maximum and curr...

Страница 44: ...t via calibration parameter menu b exhaust filter choke The PWM signal or blower speed adjustment establishes the motor speed controls curve starting point of the programmed internal reference perform...

Страница 45: ...rage 60 LFPM 5 LFPM 30 m s 025 m s b Inflow average 105 LFPM 5 LFPM 53 m s 025 m s using the direct inflow measurement method or constricted 3 inch 76mm high access opening measurement method Note It...

Страница 46: ...ease blower speed Greater than 110LFPM 56 m s First decrease blower speed to achieve the exhaust excess then close the choke plate to achieve the balance In this fashion the downflow should remain nea...

Страница 47: ...s 08 53mls Offset value may be positive or negative as needed Once desired offset value is achieved press ENT key to accept Press to exit 7 5 4 4 Alarm Verification If desired the alarm setpoint can b...

Страница 48: ...s ENT and keys sequentially releasing the key after the 3 key sequence The following service parameter display should appear Press and to select calibration Select and press ENT key for blower speed c...

Страница 49: ...m s D Acceptance Criteria 1 Average downflow velocity 60 LFPM 5 LFPM 30 m s 025 m s 2 Individual readings must be within 20 of the average downflow velocity_____ to _____ fpm _____ to _____ m s B Inf...

Страница 50: ...792 528 24 990 635 29 188 741 33 386 848 37 584 955 41 782 1061 45 980 1168 50 178 1274 54 375 1381 600E 4 102 8 158 207 12 316 313 16 474 481 20 632 524 24 790 630 28 948 735 33 106 841 37 264 946 4...

Страница 51: ...and should be removed If an air gap exhaust transition is provided the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux...

Страница 52: ...t where an audible and or visual alarm will activate to signify unfavorable operating conditions within the Biosafety cabinet and or the remote exhaust blower and canopy connection performance 7 8 1 S...

Страница 53: ...test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Ta...

Страница 54: ...2 2 AMPS 1 AMP 2 NU 543 400E 8 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 NU 543 500E 10 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 NU 543 600E 10 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 7 10 3 Main Control Board Replacement Note All setup...

Страница 55: ...The airflow sensor function utilizes two thermistors that provide a constant current source One thermistor is a reference that uses a very low current source The other thermistor is the airflow measur...

Страница 56: ...amps Check voltage coming out of main control board to light ballasts Cabinet blower won t turn on Green LED above blower key indicates the blower should be on Airflow Alarm Check blower fuse on main...

Страница 57: ...ly Menu selection moved in a round robin pattern Press ENT key on a selected menu item will either 5 Drill further down the menu tree 6 Allow editing of the item value which will now be highlighted o...

Страница 58: ...on via the outlet key If the outlet power sync is active the blower must be actively running for the outlet power to turn on 8 2 2 Function Options Disable Intelliflow If this function is active All a...

Страница 59: ...n the blower is actively running and a power interruption occurs The blower will automatically come back on when power is restored If this function is turned off the blower will not automatically come...

Страница 60: ...un time is the length of time the blower will run during and of the 4 possible blower start times Blower start schedule is the start time of 4 possible blower start times during the decon cycle Initia...

Страница 61: ...ated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally open and...

Страница 62: ...June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the...

Страница 63: ...ystem monitors UV light usage hour and when the 5000 hour point is reached The UV light LED indicator will blink fast along with the display message Replace UV light To view hours or reset timer perfo...

Страница 64: ...ep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Страница 65: ...y comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 1...

Страница 66: ...t Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative...

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