Nexcelom Bioscience Cellometer Auto T4, Руководство пользователя

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8001009 Cellometer® Auto T4 User Manual Rev C 1
Chapter 1. Introduction
This chapter presents introductory information to be reviewed prior to unpacking your Cellometer® Auto T4
instrument. It includes a product overview, lists contents of shipping container, identifies symbols/abbreviations
used on both the device label and instrument, and provides a summary of topics contained in this user manual.
AUTO T4 SYSTEM OVERVIEW
Cellometer® Automated Cell Counters incorporate the basic principles of Imaging Cytometry traditionally used in
manual cell counting via hemocytometer into a suite of instruments designed to simplify the cell counting process.
By capturing multiple images of the cells to be counted, users can adjust parameters based on cell morphology and
perform reanalysis as necessary. Cellometer
Cell Counting Chambers are equivalent to all four corner squares of a
hemocytometer and are disposable, thus saving time and effort, and eliminating any risk of cross-contamination.
Using Brightfield imaging and pattern-recognition software, the Cellometer Auto T4 quickly identifies individual
cells and accurately calculates
Cell Count , Mean Diameter (micron) and Cell Concentration (cells/mL) . Customizable
cell type parameters are designed to assist with the declustering of clumpy cell lines. For cultured cells stained with
trypan blue, the Auto T4 simultaneously calculates
Live/Dead and Total cell counts, Viability (%) , and Live and Total
cell concentrations, including a helpful graphic overlay that may be used to highlight counted live and dead cells
with color-coded outlines in the image being viewed.
The small footprint of the Auto T4 is designed to fit in small lab spaces and Cellometer Auto software can be
loaded on most computers. With an Operating Computer connected to the instrument via USB cable, the system
can be linked to a network for accessing external files, printers and storing count results. For laboratories that
require QC access and audit controls, optional GMP/GLP software can be added to the Auto T4 for tracking audit
details, offering multi-layer access control and allowing automatic printing of data/images using custom templates.
Intended Use
Intended use of the Auto T4 is to count clean cell lines and cultured primary cells such as CHO, Jurkat, C6, HT29,
DLD, KAT4, GHOST, MCF-7 and more. It can also provide total cell count and viability using trypan blue.
Personnel operating this instrument are encouraged to familiarize themselves with device controls and operation.
Ensure that users can identify all components associated with the instrument, perform adequate adjustments and
understand performance criteria. If issues are encountered, follow the
Troubleshooting guidelines on page 57 to
restore performance if instrument does not meet or exceed defined performance criteria.
WARNING: Use of controls or adjustments or performance of procedures other than those specified
herein or by Nexcelom Bioscience LLC. may result in a hazardous process.