Nexcelom Bioscience Cellometer Auto T4, Руководство пользователя

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Chapter 9. Cell Types
8001009 Cellometer® Auto T4 User Manual Rev C 43
Adjusting Cell Diameter Parameters
For each cell type, Cell Diameter Minimum / Maximum (in microns) parameter settings indicate the size of cells to
be included in the count
.
To determine if cell diameter parameters are set up properly, use the Counted check box in the Home screen to
display a graphical overlay outlining counted cells (e.g., green for counted cells and yellow for cells not counted as
they are larger than the specified cell diameter). For example, the figure below shows counted cells for the same
HT-29 image using different cell size parameter settings.
HT-29 cell count results were analyzed using different cell diameter parameter settings. Figure (a)
–
"Cell Diameter
Minimum" size is too large. Cells smaller than 15 µm are not counted, as indicated by missing outlines. Figure (b)
–
"Cell
Diameter Maximum" is too small. Cells larger than 16 µm are not counted, as indicated by the yellow outlines. Figure (c)
–
Both "Cell Diameter Minimum" and "Cell Diameter Maximum" are set up properly to include all cells. Figures (d), (e) and (f)
are respective cell size histograms for (a), (b), and (c). They are also used to establish cell size parameter settings.