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BIOFLO III

THE MICROPROCESSOR

CONTROLLED

LABORATORY-SCALE

FERMENTOR

MANUAL NO.: M1226-0050/N

NEW BRUNSWICK SCIENTIFIC CO., INC.

BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200
TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO
INTERNET: http://www.nbsc.com/ . E-MAIL: [email protected]

Содержание BIOFLO III

Страница 1: ...TORY SCALE FERMENTOR MANUAL NO M1226 0050 N NEW BRUNSWICK SCIENTIFIC CO INC BOX 4005 44 TALMADGE ROAD EDISON NJ 08818 4005 732 287 1200 TELEPHONE 800 631 5417 FAX 732 287 4222 TELEX 4753012 NBSCO INTE...

Страница 2: ......

Страница 3: ...or replacing the instrument part thereof which shall within one year after date of shipment prove to be defective after our examination This warranty does not extend to any NBS products which have bee...

Страница 4: ...UIRED PLEASE CALL OUR SERVICE DEPARTMENT AT 1 800 237 2298 PLEASE DO NOT RETURN ANY EQUIPMENT FOR SERVICE WITHOUT A RETURN AUTHORIZATION AND NUMBER WHICH CAN BE OBTAINED FROM OUR SERVICE DEPARTMENT TH...

Страница 5: ...ervice Connections 2 5 Recorder Connections CHAPTER 3 PREPARATION AND OPERATION 3 1 Cleaning of Vessel 3 2 Vessel Assembly 3 3 Preparation Before Sterilization 3 4 Sterilization Procedure 3 5 pH Probe...

Страница 6: ...4 8 Electrostatic Device ESD Warning 4 9 ESD Precautions APPENDICES I BioFlo III Operational Tips II Communication Protocols for NBS RS 232 422 DRAWING LIST...

Страница 7: ......

Страница 8: ...Four sterilizable polypropylene compression ports are provided in the glass wall for the addition of antifoam and nutrients as well as for vessel overflow in continuous culture studies Ports are prov...

Страница 9: ...M1226 0050 2...

Страница 10: ...eadplate The controller operates the antifoam addition pump that adds chemical defoamer through the port in the wall of the vessel Fig 1 Sensitivity and time adjustments are made on potentiometers loc...

Страница 11: ...e precise means of indicating flow rates in fluid systems Their design is based on a variable area principle BioFlo III is equipped with a 0 2 to 4 0 SLPM flowmeter NOTE 5L Unit uses an interchangeabl...

Страница 12: ...itch to raise or lower the set point of the parameter selected Also used during calibration of pH and DO Selector Switch to select pH DO agitation temperature or nutrient readings to be displayed Mode...

Страница 13: ...he bottom switch position AUTO is for automatic pump operation The center position is OFF External nutrient pump control Fig 7 The 6 lug terminal strip allows for external control of the nutrient pump...

Страница 14: ...agitation switches to their off position 4 Check the specification plate on the rear of the unit and plug the line cord into a suitable electrical outlet 2 4 SERVICE CONNECTIONS Water 20 max PSIG 50 m...

Страница 15: ...the Headplate Fig 9 2 Insert the sparger tube in the sparger port Fig 10 3 Insert the harvest tube in the harvest port Fig 11 Working From the Top of the Headplate 4 Insert the thermowell tube into t...

Страница 16: ...t be free of glycerin 3 3 PREPARATION BEFORE STERILIZATION Before sterilization perform the following procedure 1 Fill vessel with water to its working volume 2 Place the vessel assembly on the three...

Страница 17: ...riod of time 12 Check that the machine functions properly 13 Pull out the pH probe from the pH port and calibrate it see Section 3 6 3 4 STERILIZATION PROCEDURE 1 Remove the motor from the top of the...

Страница 18: ...T MUST BE VENTED AT ALL TIMES DURING STERILIZATION OF THE GLASS REACTOR VESSEL CONTENTS AND ELECTRODES IT IS IMPORTANT THAT THE PRESSURE BUILT UP IN THE AUTOCLAVE IS RELEASED ONLY WHEN TEMPERATURE HAS...

Страница 19: ...ode to probe cable Turn the agitation switch OFF Turn ON power switch NOTE The pH measuring system is calibrated using two buffer solutions of a known pH 1 Set the selector switch to pH Set the mode s...

Страница 20: ...parge Nitrogen into the vessel until the display is stable for approximately 5 minutes Set the mode switch to the ZERO position With the INC DEC switch adjust the display for zero reading 2 Set the SP...

Страница 21: ...sel base and the exhaust condenser with water lines 2 Carefully place the DC motor on the vessel assembly 3 Add glycerin to the thermowell and insert RTD temperature probe 4 Turn power to ON position...

Страница 22: ...t up and use D O Mode proceed as follows 1 Set selector to Agitation 2 Set mode to set point 3 Adjust rpm with Inc Dec to the minimum rpm value you wish for D O control 4 Press D O control active and...

Страница 23: ...gasket Use aseptic techniques 7 Repeat step 1 through 6 until desired number of samples are taken 3 12 SHUT DOWN PROCEDURES To shut down the system proceed as follows 1 Rotate flowmeter dial to zero 2...

Страница 24: ...tions with all switches OFF and incoming power disconnected 1 Check the fuse s for clean contact 2 Check all controls and accessible items switches knobs fuse holders screws nuts and bolts to make sur...

Страница 25: ...860 0130 DO Probe 1 25L Phoenix P0720 5440 DO Probe 2 5L Phoenix P0720 5450 DO Probe 5L Phoenix P0720 5460 DO Probe Cable P0720 2202 DO Probe 1 25L Ingold P0720 5565 DO Probe 2 5L Ingold P0720 5561 DO...

Страница 26: ...ame Same DO Port P0280 5932 Same Same DO Port Ingold Washer M1016 0890 Same Same Teflon Washer Ingold DO P0100 9780 Same Same O Ring Ingold DO Probe P0280 9163 Same Same pH P0280 5912 Same Same 3 16 P...

Страница 27: ...lowing through vessel EXHAUST CONDENSER 1 DESCRIPTION There are two quick connects on the condenser body the lower one has valve the upper one is without valve To connect the water lines connect the u...

Страница 28: ...he antifoam pump is now on the bottom and the acid pump is on the top Switching pumps is achieved by switching electrical connections in the back of the unit Simply exchange the 4 plugs which go to on...

Страница 29: ...terms have constants which are used in each term of the equation These constants have been picked based on New Brunswick Scientific s experience with this product and several cell lines The integral c...

Страница 30: ...accurate control it became apparent that the ratio of flow rate or concentration to bioreactor volume was too high In this case control to 0 12 unit was obtained If the flow rate is increased still fu...

Страница 31: ...ing and cooling times properly Most times this can be cured or improved by increasing the agitation and or airflow rates and thus improving the heat transfer Improper temperature control might also ha...

Страница 32: ...Mode The fermentor responds to inquiry and modify set points commands Multi Drop Mode The fermentor responds to inquiry and modify set points commands which are prefixed with a unit number When using...

Страница 33: ...for D and 0D is the code for carriage return The response of unit 1 to this inquiry is shown in Figure 1 0d A directive to change the setpoints of unit 1 is shown in Figure 1 0h EOL CHARACTERS The EOL...

Страница 34: ...arly Figure 1 0c shows a typical response to a request for operating points ONE DIRECTIVE IS SUPPORTED BY THE FERMENTOR Modify Setpoints Figure 1 0g shows the required format for a Modify Set points d...

Страница 35: ...FF 3 S1 4 is used for setting mode Mode S1 4 multidrop ON non multidrop OFF 4 S1 8 S1 7 S1 6 S1 5 are used for setting unit number Unit S1 8 S1 7 S1 6 S1 5 0 ON ON ON ON 1 ON ON ON OFF 2 ON ON OFF ON...

Страница 36: ..._____________ multidrop parity adata parity bit __________________________________________________ multidrop no parity adata 1 __________________________________________________ non multidrop parity p...

Страница 37: ...M1226 0050 30...

Страница 38: ...RS232 Data Input to Fermentor 4 NC 5 NC 6 NC 7 GND Ground Reference for all Signal 8 NC 9 NC 10 NC 22 NC 11 NC 23 NC 12 IRXD RS422 Paired Data Input 24 IRXD to Fermentor 13 ITXD RS422 Paired Data Out...

Страница 39: ...TABLE 5 HEXADECIMAL CODES FOR MULTIDROP UNIT NUMBERS Unit Number Hexadecimal Code 0 80 1 81 2 82 3 83 4 84 5 85 6 86 7 87 8 88 9 89 10 8A 11 8B 12 8C 13 8D 14 8E 15 8F M1226 0050 32...

Страница 40: ...M1226 0050 33...

Страница 41: ...1226 9206 Headplate 1 6c M1226 9208 Headplate 1 7a M1151 9905 Bumper Flat Gasket 2 7b M1152 9904 Bumper Flat Gasket 2 7c M1155 9902 Bumper Flat Gasket 2 8a M1151 9437 Clamping Ring 1 8b M1226 9426 Cla...

Страница 42: ...ug 1 4 3 26 M1132 946T Ferrule 1 4 3 27 P0280 5302 O Ring 4 28 M1132 9433 Plug pH 1 29 M1132 9431 Cap 1 30 P0280 5912 O Ring 2 31 M1226 9418 Plug 1 2 1 32 H 727 Plug DO 1 33 P0280 5932 O Ring 1 34 M11...

Страница 43: ...l Thermister 1 50 P0280 5362 O Ring 1 56 P0240 8270 Nut 1 57 P0240 7970 Ferrule 1 58 P0240 8260 Nut 1 59 P0240 7960 Ferrule 1 60 P0240 8240 Nut 3 61 P0240 7940 Ferrule 3 62 P0240 8230 Nut 1 63 P0240 7...

Страница 44: ...ulation port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 fle...

Страница 45: ...ulation port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 fle...

Страница 46: ...port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 flexible h...

Страница 47: ...nstallation 17 DO Probe Phoenix Installation 18 DO Probe Ingold Installation 19 Conversion of DO Port to Inoculation Port 20 Exhaust Condenser Installation 21 Restrictor in Condenser Water Line 22 Dis...

Страница 48: ...FIGURE 1 VESSEL SCHEMATIC M1226 0050 41...

Страница 49: ...FIGURE 2 SAMPLING SYSTEM M1226 0050 42...

Страница 50: ...FIGURE 3 FLOWMETER CONVERSION M1226 0050 43...

Страница 51: ...FIGURE 4 FRONT PANEL M1226 0050 44...

Страница 52: ...FIGURE 5 REAR OF CABINET M1226 0050 45...

Страница 53: ...FIGURE 6 SWITCH PANEL M1226 0050 46...

Страница 54: ...FIGURE 7 EXTERNAL NUTRIENT PUMP TERMINAL M1226 0050 47...

Страница 55: ...FIGURE 8 SUGGESTED IMPELLER LOCATION M1226 0050 48...

Страница 56: ...FIGURE 9 HEADPLATE M1226 0050 49...

Страница 57: ...FIGURE 10 SPARGER TUBE M1226 0050 50...

Страница 58: ...FIGURE 11 HARVEST TUBE M1226 0050 51...

Страница 59: ...FIGURE 12 THERMOWELL M1226 0050 52...

Страница 60: ...FIGURE 13 FOAM PROBE M1226 0050 53...

Страница 61: ...FIGURE 14 BAFFLE INSTALLATION M1226 0050 54...

Страница 62: ...FIGURE 15 SIDE PORTS M1226 0050 55...

Страница 63: ...FIGURE 16 pH PROBE INSTALLATION M1226 0050 56...

Страница 64: ...FIGURE 17 D O PROBE PHOENIX INSTALLATION M1226 0050 57...

Страница 65: ...FIGURE 18 D O PROBE INGOLD INSTALLATION M1226 0050 58...

Страница 66: ...FIGURE 19 CONVERSION OF D O PORT TO INOCULATION PORT M1226 0050 59...

Страница 67: ...FIGURE 20 EXHAUST CONDENSER INSTALLATION M1226 0050 60...

Страница 68: ...FIGURE 21 RESTRICTOR IN CONDENSER WATER IN LINE M1226 0050 61...

Страница 69: ...FIGURE 22 DISASSEMBLY OF CONDENSER M1226 0050 62...

Страница 70: ...FIGURE 23 FACTOR AFFECTING pH CONTROL M1226 0050 63...

Страница 71: ...J803 J804 J805 ADDRESS 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 9 10 11 8 7 4 5 1 2 3 RTDI RECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5...

Страница 72: ...C B M1151 7008 J802 J801 J806 J803 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEP J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M11...

Страница 73: ...AGIT ON MODE S001 J1 NC J2 1 2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE...

Страница 74: ...OFF SI 8 1 3 2 0 ON ON ON ON 4800 9600 SI 6 SI 7 ON ON OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF SHIELD J1 3 PUMP M 100 VAC ON PRIME S009 D C 2 TB002 B A C VALVE TB002...

Страница 75: ...7002 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH S...

Страница 76: ...R M1151 4011 9 10 11 8 7 4 5 1 2 3 RTDI RECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 70...

Страница 77: ...51 7008 J802 J801 J806 J803 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEPT J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009...

Страница 78: ...S001 J1 NC J2 1 2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE RS232C MODE...

Страница 79: ...800 9600 SI 6 SI 7 ON ON OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF CIRCULATION PUMP M SHIELD J1 3 WHT W BLK BLK C TB002 115 VAC PRIME S009 WHT VALVE TB002 BRN ON A B D...

Страница 80: ...SE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3...

Страница 81: ...ECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009 D O pH TRANSMITTER OFF OFF OFF OFF OFF...

Страница 82: ...03 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEPT J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009 D O pH TRANSMITTE TB001...

Страница 83: ...2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE RS232C MODE CONTROL 2 1 SET...

Страница 84: ...OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF CIRCULATION PUMP M SHIELD J1 3 WHT W BLK BLK C TB002 220 VAC PRIME S009 WHT VALVE TB002 BRN ON A B D C 2 4 W BLK BLK TB001 P0...

Страница 85: ...6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTO...

Страница 86: ...M1226 0050 79...

Страница 87: ...FIGURE 25 BEARING HOUSING ASSEMBLY M1226 0050 80...

Страница 88: ...FIGURE 26 BEARING HOUSING IN STERILIZATION M1226 0050 81...

Страница 89: ...M1226 0050 82...

Страница 90: ...M1226 0050 83...

Страница 91: ...M1226 0050 84...

Страница 92: ...M1226 0050 85...

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