NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
82
11. Apply sample to the sample window and press the sample button to
initiate the measurement.
C
ALCULATIONS
The absorbance ratio is calculated from the two path lengths specified by the user in the
parameters.
λ
1
:
λ
2
=
λ
1
λ
2
λ
1
:
λ
2
= Absorbance Ratio
λ
1
=
Absorbance 1 corresponding absorbance value 1 selected normalized to 10 mm path
λ
2
=
Absorbance 2 corresponding absorbance value 2 selected normalized to 10 mm path
M
ORE
A
PPS
:
C
ONCENTRATION
M
ETHOD
O
VERVIEW
In this mode, concentration can be calculated for a sample by determining the absorbance at a
specific wavelength relative to a reference. The concentration is then obtained by multiplying the
measured absorbance by a specific factor. This factor may be known in advance and entered by
the user, or it may be calculated by the instrument by measuring a set of standard (standard
curve method) with known concentrations to create a standard curve.
M
EASUREMENTS
P
ROTOCOL
1. Select the More Apps icon from the home screen and the
Concentration icon from the More Apps screen.
2.
To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3.
For NanoVolume application:
Select the dilution depending on the sample concentration.
