ADAM-CellT, A New Standard of Automated Cell Counter
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Introduction
Technology
Until now, cell counting and viability measurement for many types of cells
have been performed manually using hemocytometer with Trypan Blue
exclusion method, which is to distinguish viable cells from non-viable cells.
One drawback of this method, however, is the propensity for the staining
of artifacts; another drawback is that the naked eye can only differenti-
ate between cells in a limited concentration range in the hemocytometer
chamber. This combined with the potential problem of cell aggregation
and limited sample volume leads to the common variation of counts nor-
mally associated with this method.
To address these problems, NanoEntek has developed the ADAM-CellT,
which is based on a fluorescent microscopy technique for counting cells.
The ADAM-CellT utilizes sensitive fluorescence dye staining, LED optics and
CMOS detection technologies to make the cell analysis more accurate and
reliable.
To count cells using ADAM-CellT, the cells are mixed with Propidium Iodide
(PI) stain/ Acridine Orange (AO) and directly pipetted on to a disposable
plastic chip. The chip is then loaded onto a precision stage. An ADAM-CellT
system is automatically focused onto the chip and cells that have been
stained are recorded by a sensitive CMOS camera. The image results are
automatically processed generating the cell count which is displayed on
the front of the instrument. Simple. Fast. Accurate. Reliable.
AccuChip
CMOS Camera
Emission Filter
Dichroic Filter
Objective Lens
Excitation filter
Lenses
Green LED
Mirror