NanoEnTek ADAM CellT, Инструкция по эксплуатации, Страница: 16 / 57

16
Introduction
T1
N1
T2
N2
When you load of the sample mixture to the AccuChip, please be careful
not to make bubbles. ! NOTE
Concentration out of this range will result in errors.
Refer to page 21 for more information about errors. ! NOTE
Counting cell
General Operation
Sample
preparation
3. Thoroughly mix the cell pellet by vortexing.
4. Check visually if any cell clumps or agglomerates are remained.
[AccuChip 2x]
[AccuChip 4x]
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
T .
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on T channel. Then, wait
1 minute for the sample settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
N .
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on N channel. Then, wait
1 minute for the sample settling.
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
T .
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on T1 or T2 channel.
Then, wait 1 minute for the sample
settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution
N .
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on N1 or N2 channel.
Then, wait 1 minute for the sample
settling.
Total
Total
T/N1 T/N2
Non-viable
Non-viable
1. Cultivate the required number of cells.
2. Add an appropriate volume of growth media or PBS to dilute to a final
concentration of 5 x 10
4
cells/mL to 4 x 10
6
cells/mL (T
PI
/N solution).
When using T
AO
/N solution, prepare to a final concentration of 5 x 10
4
cells/mL to 2 x 10 7 cells/mL.