NanoEnTek ADAM CellT Скачать руководство пользователя страница 14 | Manualshive

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NanoEnTek ADAM CellT Скачать руководство пользователя страница 14

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Introduction

Getting Started

Count setting

Set the conditions in the ‘Setting’ tap before counting.

[AccuChip]

Set the AccuChip according to you are using.

[Cell size]

Set the minimum and maximum size of cell.

[Dilution factor]

When diluting sample, set the Dilution factor.

Factor values for the AccuStain Solution is already applied.

!

CAUTION

2Ch

4Ch

Accuchip

4Ch

2Ch

Accuchip

Cell size

Min

5

Max

80

Dilution factor

T1

N1

T2

N2

1.0

[Solution type]

Set the AccuStain Solution type (Propidium iodide (PI) or Acridine orange (AO))

T

PI

/N

T

AO

/N

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Содержание ADAM CellT

Страница 1: ...Instruction Manual A New Standard of Automated Cell Counter Copyright NanoEntek Inc All rights reserved ADAM...

Страница 2: ......

Страница 3: ...ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649...

Страница 4: ......

Страница 5: ...st Safety precautions Safety symbols Warnings Technical Support General Operation Introduction Sample preparation Counting cell Measure Run sample Result analysis Result analysis Error code Data QC mo...

Страница 6: ...6 Introduction General Description The ADAM CellT is a benchtop automated cell counter designed to perform cell counting and viability measurements using AccuStain Solution...

Страница 7: ...ddress these problems NanoEntek has developed the ADAM CellT which is based on a fluorescent microscopy technique for counting cells The ADAM CellT utilizes sensitive fluorescence dye staining LED opt...

Страница 8: ...stained AO included in Solution TAO is permeable dye which permeates cell membrane and stains DNA Regardless of the condition of the cell membrane or active metabolism AO can stain both viable cells a...

Страница 9: ...t room temperature AccuStain Solution should be stored at 2 8 C Item Quantity Main device 1 Power cord 1 USB hub 1 Adapter 1 User s manual 1 Support for 21 CFR Part 11 Compliance 1 AccuChip Kit 1 USB...

Страница 10: ...cted Power Power on off LCD Display processes and results START Performs all procedures of automatic counting LOCK Protects the alignment of stage from external shock when ADAM CellT is being moved Lo...

Страница 11: ...r view of ADAM CellT is shown below Port Description USB Port Port for software update and save the data PC port Connects with PC Power Plug Connects ADAM CellT power cord to wall outlet Product Descr...

Страница 12: ...7 Ensure minimal contact with dust or other airborne particles 8 Allow a 10 cm 4 inches minimum space around the device for proper airflow 9 Do not place any objects on the device 1 Check the connecti...

Страница 13: ...lure of X axis sensor 0x00007000 Failure of Y axis sensor 0x00008000 Failure of Z axis sensor 0x06000000 Failure of Locking module sensor Getting Started It appears when booting is not working properl...

Страница 14: ...e Set the minimum and maximum size of cell Dilution factor When diluting sample set the Dilution factor Factor values for the AccuStain Solution is already applied CAUTION 2Ch 4Ch Accuchip 4Ch 2Ch Acc...

Страница 15: ...mated cell count using the ADAM CellT Please check the procedure of sample preparation and testing below For more detailed information please refer to the next page 2 Load the mixed sample Then wait 1...

Страница 16: ...Solution N 2 Voltex the tube vigorously 3 Load 23 L sample mixture to the AccuChip on N channel Then wait 1 minute for the sample settling Total Cell Non viable Cell 1 Add 50 L of your sample to 50 L...

Страница 17: ...2 T1 N1 T2 N2 General Operation Counting cell Correct volume Low volume Sample loading error Be cautious of loading the correct volume of the sample into AccuChip The instrument will not detect low or...

Страница 18: ...The instrument will not detect if slides are inserted incorrectly See pictures below for proper insertion WARNING PleaseinsertorremovetheAccuChipwhentheslideholderisfullyejected Whenthetestisfinished...

Страница 19: ...d Cell Counter 19 Introduction Measure Run Sample Start counting process by pressing START It may take about 2 minutes longer for auto focus at the initial test While the test is in progress you can c...

Страница 20: ...5 75 The viability will be automatically calculated by the ADAM CellT software after each measurement of the total cells and the non viable cells First the total cell number and second non viable cell...

Страница 21: ...meter is larger than 100 m When this error shown in result window please check the image Error code Cause E Frames with errors are over 50 of total counting frame O Cells are more than 2X107 cells mL...

Страница 22: ...Show Data Displays the date of QC progress on the graph QC Result Graph Graph left right QC date number movement button Arrow Button Graph left right QC date number movement button QC Slide QC Slide L...

Страница 23: ...tered QC Slide lot information list New Registers new QC Slide lot Edit Edits selected QC Slide lot Delete Deletes selected QC Slide lot Apply Close Applies function or closes selected QC Slide lot Lo...

Страница 24: ...ll Selects all QC result to delete from the QC result List QC Result List QC result information list Visible Selects all QC result to display graph from the QC result List Delete Deletes selected QC r...

Страница 25: ...e different for each QC Slide lot and the acceptance slide peak size 13 16 um The acceptance range of the selected QC slide lot is displayed on the graph as a purple area Acceptance slide peak size re...

Страница 26: ...edit the data Multiple data can be edited with the same settings IMAGE Allows to check the cell images of each channel SAVE Saves the selected data to USB PRINT optional Prints the selected data MAIL...

Страница 27: ...w the cell size graph each channel T N Cell size table Allows to view the number of cells in each cell size Channel Select a channel T N Cell size setting Set the min max size of the cell Dilution Fac...

Страница 28: ...AGE Control buttons Description Channel Select a channel Original Check the original image Counted Check the counted cell image Frame Select a frame number of the channel Zoom in out Zoom in and out t...

Страница 29: ...oduction Data SAVE Control buttons Description Select Path Selects a save path from the list to send the selected data Data Type Selects which data type to save Apply Exports the files to a selected s...

Страница 30: ...to send data The e mail address where data has been sent will be saved Mail Address To send files to new e mail enter the applicable e mail address Data Type Selects which data type to send via e mai...

Страница 31: ...approval cannot be edited or deleted Approved data cannot be edited NOTE Approval Approval Control buttons Description Approval Status Settings tab related to data approval such as Requestable Reques...

Страница 32: ...Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of the selected date Cancel Cancel approv...

Страница 33: ...proval such as Requestable Requesting Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of t...

Страница 34: ...al such as Requestable Requesting Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of the s...

Страница 35: ...mote support software Mail DO NOT change the setting in mail Number Selects auto numbering Update Updates firmware or software through USB Auto Logout Sets auto logout time Date Time Sets current date...

Страница 36: ...lick Remote support button 3 Share your ID and password to NanoEntek If connection error occurs please contact a laboratory facility manager CAUTION The remote support feature is to be used for mainte...

Страница 37: ...repare the USB with update file 2 Insert the USB 3 Click the UPDATE button The AdamUpdate foldermustexistintherootpathoftheUSBfolder ADAM CellTcanbeupdatedonlywhenthefirmwareorsoftwarefileexistsin the...

Страница 38: ...ckup Sync Manual recovery restores manually backup counted data Setting The automatic backup function is turned off after recovery please re enable the automatic backup function NOTE Please be careful...

Страница 39: ...To logout User manage To register user Log manage Tracks user access records Document manage Tracks management document records Deleted list Tracks deleted data records ADAM CellTprovidesacomprehensi...

Страница 40: ...iption User Name To view the registered user list Permission To view the user access authority New Registration To register new user Privilege Option for permission setting Supervisor Permission To re...

Страница 41: ...ings Function list Detailed functions for each permission setting tab Detailed function List of possible permission settings for each function The Admin can grant or release access to functions when c...

Страница 42: ...ount lock Number of incorrect passwords in account lockout 3 5 10 15 Minimum length Minimum length of password 3 5 10 15 Reuse Prohibition of using the same password for a certain period of time 30 90...

Страница 43: ...l Counter 43 Introduction User Log manage Control buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the...

Страница 44: ...ol buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the selected data CLOSE Close the document manage...

Страница 45: ...Counter 45 Introduction Control buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the selected data CLO...

Страница 46: ...on for 3 minutes the lock function will be activated automatically When the device is locked the screen will be changed as shown below If you press the power button for 2 3 seconds then Slide to shut...

Страница 47: ...llT has no replacement of consumable materials 3 Please clean the exposed surface of ADAM CellT frequently or before testing using a soft cloth and isopropyl alcohol or deionized water Dispose of wipe...

Страница 48: ...ion on each error message see page 21 When frames with errors are over 50 of total counting frame Error message E Check the suspension of cells if all cells are fully dissociated into single cells If...

Страница 49: ...rts supplied by anyone other than NanoEntek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection items being returned...

Страница 50: ...AO PI 4x105 2x106 cells mL PI 4x105 1x107 cells mL AO PI 25 50 sec test DC12V 5A 4 X 4W Green LED CMOS camera Excitation filter Dichroic filter Emission filter 7 Kg 227 276 270 mm IPX0 23 L test Accu...

Страница 51: ...uchip 4x Kit AO PI 200 pcs AccuChip 4X 1 12 5 mL AccuStain Solution T TAO 2 12 5 mL AccuStain Solution N 1 ADR 1000 Accustain Solution PI solution 12 5 mL AccuStain Solution T TPI 4 12 5 mL AccuStain...

Страница 52: ...objects especially metallic into the air vents of the instru ment as this could result in electrical shock personal injury and equip ment damage Always set the main switch on the power supply unit to...

Страница 53: ...quirements Instruments bearing the TUV symbol are certi fied by TUV SUD America Inc to be in conformance with the applicable safety standard for the US and Canada LED USB Connection When this crossed...

Страница 54: ...erson Cover Do not remove cover or dissemble case There are no adjustable components inside the instrument If a malfunction is found refer to an authorized service person Manual Do not attempt to serv...

Страница 55: ...c Technical support contact information Additional product information and special offers NanoEntek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 62...

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Страница 57: ...40 Fax 82 2 6220 7999 NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 EC Representative MT Promedt Consulting GmbH Ernst Heckel Stra e 7 6...

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