Chapter 5: Read Modes and Read Types
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The kinetic read type can collect data points in time intervals of seconds, minutes, or hours
(up to 99 hours).
Kinetic analysis has many advantages to determine the relative activity of an enzyme in
different types of plate assays, including ELISAs and the purification and characterization of
enzymes and enzyme conjugates. Kinetic analysis is capable of providing improved dynamic
range, precision, and sensitivity relative to endpoint analysis.
Spectrum
The Spectrum read type measures optical density (OD) or %Transmittance across a specified
wavelength range, with allowed values from 200 nm to 1000 nm. For the Fluorescence or
Luminescence read mode, relative fluorescence units (RFU) or relative luminescence units
(RLU) values are reported.
Well Scan
The Well Scan read type takes reads at more than one location on an evenly spaced, user
defined grid or other pattern inside of each well at single or multiple wavelengths. The fill
pattern is either round or square to match the well shape. The number of points that are
available depend on the well size of the plate you select. Four scan patterns are available:
