Appendix A: Specifications
0112-0102 E
47
Item
Description
Sensitivity
Top Read (M2 and M2e)
3.0 fmol/well in 200 µL FITC (96 well microplate and 15 pM)
3.0 fmol/well in 75 µL FITC (384 well microplate and 40 pM)
Bottom Read (M2e only)
5.0 fmol/well in 200 µL FITC (96 well microplate and 25 pM)
5.0 fmol/well in 75 µL FITC (384 well microplate and 67 pM)
Sensitivity (cuvette)
< 15 pM fluorescein
Excitation wavelength range
250-850 nm
Emission wavelength range
360-850 nm (M2)
250-850 nm (M2e)
Wavelength selection
Monochromators, tunable in 1 nm increments
Bandwidth (excitation, emission) 9 nm, 9 nm
Number of excitation/emission
pairs per plate
4
Dynamic range
10
6
in 96-well black plates: auto gain circuitry
System validation
Self-calibrating with built-in fluorescence calibrators
Light source
Xenon flash lamp (1 joule/flash)
Average lamp lifetime
1 billion flashes
Detector
Photomultiplier (R3896)
Table A-2: Fluorescence Intensity Performance