Molecular Devices SpectraMax GEMINI EM, Руководство оператора

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Instrument Description
SpectraMax GEMINI EM Operator’s Manual 1-17
Incubator
Choosing
Incubator
from the
Control
menu or clicking the incubator button
opens a dialog box allowing you to start or stop temperature regulation and
to select an elevated temperature for the microplate chamber.
Instrument Setup
(In SoftMax Pro software) De fi nes the parameters (mode, wavelengths, auto-
matic mixing, run time, read interval, etc.) used to read the microplate.
Kinetic
During kinetic readings, data is collected over time, with multiple readings
made at regular intervals. The values calculated based on raw kinetic data are
Vmax, Time to Vmax, and Onset Time. Kinetic readings can be single- or mul-
tiple-wavelength readings.
LCD (Liquid Crystal Display)
A 2-
×
-20-character display which shows the current instrument settings.
Luminescence
The emission of light by processes that derive energy from essentially non-
thermal changes, the motion of subatomic particles, or the excitation of an
atomic system by radiation.
Parallel Port (Windows)
A connection on a computer, usually LPT1, where you plug in the cable for a
parallel printer. Windows supports parallel ports LPT1 through LPT3.
Photomultiplier Tube (PMT)
A vacuum tube that detects light especially from dim sources through the use
of photoemission and successive instances of secondary emission to produce
enough electrons to generate a useful current.
Read Mode
The method used to read the microplate: endpoint, kinetic, spectrum, or well
scan.
Read Type
The type of reading performed: fl uorescence or luminescence.
Readings per Well
The number of times (user-de fi nable) that readings are taken on a well in fl uo-
rescence mode or the amount of time that data is collected using the lumines-
cence read type.
SoftMax Pro
An integrated software program (from Molecular Devices Corporation) that is
used to control and collect data from the SpectraMax GEMINI EM instru-
ment.
Stokes Shift
The difference between the wavelengths of the excitation and emission peaks.