Molecular Devices SpectraMax GEMINI EM, Руководство оператора

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Instrument Description
SpectraMax GEMINI EM Operator’s Manual 1-13
Temperature regulation and control of the microplate chamber is achieved
through electric heaters, a fan, ef
fi cient insulation, and temperature sensors.
The heaters are located in the microplate chamber which is insulated to main-
tain the temperature set point. The sensors are mounted inside the chamber
and measure the air temperature.
The temperature feedback closed-loop control algorithms measure the cham-
ber air temperature, compare it to the temperature set point, and use the dif-
ference to calculate the regulation of the heating cycles. This technique results
in accurate, precise control of the microplate chamber temperature.
Automix
The Automix function permits automatic shaking of the microplate at preset
intervals, thereby mixing of the contents within each well. Automix must be
selected before beginning a reading. The actions associated with the Automix
setting depend on the read mode chosen.
For endpoint mode, enabling Automix will shake the plate for a de fi nable
number of seconds and then read at all selected wavelengths.
When kinetic mode is chosen, two types of Automix can be enabled: you can
set Automix to shake the plate for a de
fi nable number of seconds before the
initial reading and/or for a de fi nable number of seconds before each subse-
quent reading.
Use of Automix is strongly recommended for ELlSAs and other
solid-phase, enzyme-mediated reactions to enhance accuracy.
Computer Control
The SpectraMax GEMINI EM is equipped with an 8-pin DIN RS-232 serial
port through which the computer communicates with and controls the instru-
ment. (Different types of cables are available for connecting to different types
of computers—see Appendix A, “Cables and Accessories.”) SoftMax Pro soft-
ware, version 4.1 or greater, is required to control the SpectraMax
GEMINI EM.
NOTE: