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ImageXpress Micro High Content Imaging System Options User Guide
10 5014166 D
Environment Control Hardware
The ImageXpress Environment Control option consists of a sealing ring
on top of the sample plate and a top door above the plate that together
forms a small, sealed volume. Humidified carbon dioxide is sourced into
this small volume to form the required environment above the plate.
Temperature is controlled within the upper half of the instrument.
The ImageXpress Environment Control option consists of the following
hardware subsystems:
• Temperature control within the upper half of the base
instrument. Warm air is provided from the Environmental
Control Option Controller or the Systems Power & Options
Controller through an air hose. Feedback from temperature
sensors installed near the plate maintains the temperature. The
temperature can be controlled within a range of 30°C to 40°C,
when the ambient room temperature is 22°C or lower.
• Carbon dioxide is provided from a customer-supplied tank of
pre-mixed 5% CO
2
and 95% air. The tank regulator must be set
between 15 PSI and 20 PSI. The Environmental Control Option
Controller or the Systems Power & Options Controller controls
the flow to the space above the plate, maintained by the live-cell
sealing ring. If a plate is ejected and loaded, the system conducts
a purge cycle automatically.
• Humidity is passively provided by bubbling the carbon dioxide
through a water reservoir, minimizing evaporation from the
sample plate over the duration of a timelapse experiment.
Items Included in the Installation
The following hardware components are included in an ImageXpress
Environment Control option installation:
• ImageXpress Micro Environmental Control Option Controller or
the ImageXpress Micro Systems Power & Options Controller .
See Figure 1-1 on page 11 and Figure 1-2 on page 11 .
• Warm air hose and carbon dioxide tubing . See Figure 1-3 on
page 12 and Figure 1-4 on page 12 .
• Temperature sensors . To ensure accurate readings, the sensors
are located near the sample plate.
• Water reservoir . See Figure 1-5 on page 13 .
• Live-cell sealing ring . Compatible with 96-well and 384-well
standard height plates. The standard height for these plates is
14.35 mm ± 0.25 mm (0.5650 inches ± 0.0098 inches). See
Figure 1-6 on page 13 .
ImageXpressOptionsUserGuide.book Page 10 Wednesday, September 9, 2015 3:54 PM