ImageXpress Micro High Content Imaging System Options User Guide
20
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Before you run an experiment:
•
Allow the instrument to warm up for at least two hours.
•
Make sure the plate sealing ring is at 37
°
C before use, either by
keeping it inside the instrument or inside an incubator. Incorrect
sealing-ring temperature can cause temperature fluctuation.
•
Wait 30 minutes to 1 hour for the plate to reach equilibrium. You
can use a journal to set this waiting period.
The current temperature in the chamber is displayed on the
front of the controller.
•
If there is
interstitial
space between wells, pipette deionized
water or media into these areas. This helps to increase the
thermal mass of the plate and reduces overall evaporation.
1.
Note:
Due to the changes in Z height over time, you might need
to adjust the plate focus parameters in the MetaXpress software
occasionally. To do so, open the MetaXpress software, and then
click
Screening
>
Plate Acquisition Setup
>
Plate tab
>
Edit Plate
Bottom Settings
. Increase the adjacent well max variation to
accommodate the added Z-height variation introduced by the
fluctuation of temperature.
When setting laser autofocus options, make sure that the
instrument and plate temperatures are at equilibrium. Room-
temperature laser autofocus settings are not ideal when the
system is used at higher temperatures.
ImageXpressOptionsUserGuide.book Page 20 Wednesday, September 9, 2015 3:54 PM
