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Luminex Luminex 200, Руководство пользователя системы

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Luminex 200 

System User Manual

xMAP Technology Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Maintenance and Cleaning. . . . . . . . . . . . . . .  17

Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Before Running Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
After Running Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Routine Tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Sheath and Waste Fluids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Weekly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Visual Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Clean the Sample Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Flush the System  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Monthly  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Clean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Calibrate and Verify the System  . . . . . . . . . . . . . . . . . . . . . . . . 21
Clean the Sample Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Every Six Months . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Luminex 200 Analyzer Air Intake Filter . . . . . . . . . . . . . . . . . . 22
Luminex XYP Instrument Air Intake Filter. . . . . . . . . . . . . . . . 23
Syringe Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Luminex 200 Analyzer Ventilation Filter . . . . . . . . . . . . . . . . . 26

Annually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Sheath Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

As required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Fuses  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Replacing the SD System with a Sheath Bottle . . . . . . . . . . . . . 29
Storing the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Taking the System out of Storage . . . . . . . . . . . . . . . . . . . . . . . 30

Maintenance Log  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . .  33

Troubleshooting the Luminex 200 System. . . . . . . . . . . . . . . . . . . . 33
Power Supply Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Communication  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fluid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Sample Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Calibration and Control Problems  . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Acquisition Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Bead Detail Irregularities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Luminex SD Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Malfunction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Draining the Reservoir. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Product Numbers  . . . . . . . . . . . . . . . . . . . . . .  47

Содержание Luminex 200

Страница 1: ...Luminex 200 System User Manual Lum nex...

Страница 2: ...Although prepared to ensure accuracy Luminex assumes no liability for errors or omissions or for any damages resulting from the application or use of this information This guide may be updated period...

Страница 3: ...S OF BUYER OR OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON ACCOUNT OF SUCH LOSS OR FOR ANY LABOR OR ANY OTHER EXPENSE DAMAGE OR LOSS OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJURY OR...

Страница 4: ...any and all claims demands actions causes of action and or suits in law or equity now existing or hereafter arising whether known or unknown against Seller and Luminex and their respective officers d...

Страница 5: ...sale of Luminex instrumentation to you the purchaser you may obtain a license under Luminex s patents if any to use this unit of Luminex instrumentation with fluorescently labeled microsphere beads au...

Страница 6: ...RMITTED BY APPLICABLE LAW LUMINEX AND ITS SUPPLIERS DISCLAIM ALL WARRANTIES EITHER EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOS...

Страница 7: ...Shipment 6 The System 7 Theory of Operation 7 Hardware 8 xMAP Technology Reagents 8 Required Laboratory Reagents 9 Luminex Software 9 Luminex 200 Performance Specifications 9 Speed 9 Accuracy and Pre...

Страница 8: ...ake Filter 22 Luminex XYP Instrument Air Intake Filter 23 Syringe Seal 24 Luminex 200 Analyzer Ventilation Filter 26 Annually 27 Sheath Filter 27 As required 28 Fuses 28 Replacing the SD System with a...

Страница 9: ...iii Luminex 200 System User Manual Hardware 47 Software 48 xMAP Reagents 49...

Страница 10: ......

Страница 11: ...signal reader unit raw data storage mechanisms data acquisition software and software to process detected signals Warnings and Notes Informational notes and warnings appear in this manual Note A note...

Страница 12: ...ard laboratory safety practices The protection provided by the equipment may be impaired or the warranty voided if the system is used in a manner not specified by the instructions or by Luminex Corpor...

Страница 13: ...strong electromagnetic radiation for example unshielded intentional RF sources as these may interfere with proper operation Warning Always handle the Luminex 200 system according to Luminex instructi...

Страница 14: ...urn power to the Luminex 200 analyzer OFF and disconnect the power cord All laser apertures are located within the Luminex 200 analyzer and are contained within a protective housing Barcode Reader Las...

Страница 15: ...d fingers away from the Luminex XYP instrument slot during operation Warning During operation this system contains exposed moving parts which could result in pinch point hazard Risk of personal injury...

Страница 16: ...er 4 Sanitize the system using the Sanitize command in the Luminex software Follow this by washing twice with distilled water 5 Disconnect the system from AC power by turning off the power switch on t...

Страница 17: ...Fluid by the second fluidics path This process removes residual sample within the tubing valves and probe The second fluidics path is driven by positive air pressure and supplies sheath fluid to the...

Страница 18: ...analysis of the xMAP microspheres as they are processed through the Luminex 200 analyzer Results of the analyses are processed and provided in a report format Hardware The Luminex 200 system includes...

Страница 19: ...s prohibited and may result in improper operation of the system Luminex 200 Performance Specifications Speed USB communications link for fast data transfer Automatic transfer of assay templates and ne...

Страница 20: ...ue xMAP microsphere sets in a single sample Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1 100 unique xMAP microspheres sets in a single sample Sample cor...

Страница 21: ...ange 100 120 V 10 1 4 Amp and 200 240 V 10 0 8 Amp 47 63 Hz AC inlet fuse 3 Amp 250 V fast acting Optics Reporter laser 532 nm nominal output 10 15 mW maximum 500 mW frequency doubled diode mode of op...

Страница 22: ...o 86 F Humidity 20 to 80 noncondensing Altitude designed to operate at up to 2400m 7874 feet above mean sea level Physical dimensions 20 cm 8 inches W x 30 cm 11 75 inches D x 24 75 cm 9 75 inches H W...

Страница 23: ...number 08849 00 Operating frequency 55 kHz or equivalent System Overview The system consists of three subsystems electronic fluidic and optical The following section describes the user accessible comp...

Страница 24: ...s fitting when you remove the sample probe See Figure 2 5 7 4 3 2 1 6 1 Air intake filter access door 5 Communication Ports DB9 2 Power Switch 6 XYP Communication Port DB9 3 Power Input Module 7 Analy...

Страница 25: ...ess doors Two of the access doors are on the front and the third is on the back The front left access door supplies access to the sheath filter The front center access door supplies access to the syri...

Страница 26: ...the SD system sheath fluid levels must be monitored manually Check the sheath fluid level before starting a run or procedure Waste Fluid Container The waste fluid container receives waste from the sys...

Страница 27: ...ces Daily Maintenance If the system is powered on but idle for more than four hours click Warmup Wait 30 minutes for the Luminex 200 analyzer and the optics system to warm up Before Running Samples 1...

Страница 28: ...elected well For a round bottom U bottom plate stack two of the smaller 3 35 mm diameter alignment discs in the selected well For a plate with conical wells place one alignment sphere into the selecte...

Страница 29: ...o become pressurized causing pressure errors in the system If the seal gets wet let it air dry Touching the seal can contaminate it If it gets dirty you should replace the waste bottle cap If you are...

Страница 30: ...intake filter for buildup of dust Check the SD system and its connection for leaks If you see a leak turn off the power to the Luminex SD system and contact Luminex Corporation Clean the Sample Probe...

Страница 31: ...D system 2 Wipe all exterior surfaces with mild detergent then a 10 to 20 household bleach solution and finally with plain distilled water 3 Open both doors of the analyzer Clean all accessible surfac...

Страница 32: ...backflushes 3 drains 2 alcohol flushes and 3 washes with distilled water Every Six Months Luminex 200 Analyzer Air Intake Filter To replace the analyzer air intake filter 1 Disconnect the Luminex 200...

Страница 33: ...on the Luminex 200 analyzer Luminex XYP Instrument Air Intake Filter To replace the XYP instrument air intake filter 1 Disconnect the Luminex XYP instrument from AC power by turning off the power swit...

Страница 34: ...Removing and Replacing the Filter 4 Plug in and turn on Luminex XYP instrument power Syringe Seal To replace the syringe plunger seal 1 Turn the power switch on the back of the analyzer to the off po...

Страница 35: ...soon as it begins traveling upward turn off the analyzer CAUTION Do not turn off the analyzer as the arm is going down The syringe pump valve will not be in the correct position allowing sheath fluid...

Страница 36: ...ea 6 When the prime finishes close the analyzer door Luminex 200 Analyzer Ventilation Filter To clean the Luminex 200 analyzer ventilation filter 1 Disconnect the Luminex 200 analyzer from AC power by...

Страница 37: ...yzer from AC power by turning off the power switch on the rear of the analyzer then unplugging the analyzer power cord from the wall source 2 Disconnect the sheath fluid tubing before changing the fil...

Страница 38: ...the power cord from the analyzer or instrument 2 With a small flathead screwdriver open the module door on the lower left corner of the back of the analyzer or instrument See Figure 9 Figure 9 Opening...

Страница 39: ...Warm Up via the software if the compressor has turned off 8 When the pressure has stabilized note the Sheath pressure 9 If the pressure is equal to the value noted in step 2 within 0 1psi then the se...

Страница 40: ...at the instruments are responding correctly The light above the sample probe on the Luminex 200 and the light next to the door of the XY platform are lit The compressor starts in the Luminex 200 It is...

Страница 41: ...isopropanol or 70 ethanol Wash twice with distilled water Shut down Initials for each item listed at the left fill in your initials under each date that you perform the item Sanitize 10 to 20 househo...

Страница 42: ......

Страница 43: ...he following topics Power Supply Problems Communication Pressurization Fluid Leaks Sample Probe Calibration Problems Acquisition Problems Bead Detail Irregularities Printing Errors Verification Lumine...

Страница 44: ...control sample acquisition session uploading and start stop and pause features Symptom Possible problem Solution Analyzer will not turn on or XYP will not turn on The power cord is disconnected Verif...

Страница 45: ...there is fluid on the surface on which the system sits The compressor does not engage Run a Prime command If you do not hear the compressor turn on contact Technical Support The Cheminert fitting is...

Страница 46: ...24 The syringe valve leaks Hand tighten the syringe connection silver knob on the syringe valve Run a Prime command If leaks continue contact Technical Support Large amount of fluid pooled around ins...

Страница 47: ...Sample arm is stuck in the down position The sample probe height is too low or the path to the well is blocked DO NOT turn off the Luminex XYP instrument 1 Remove the blue light housing from the analy...

Страница 48: ...libration is slow or fails The calibration microspheres are not fully suspended Vortex the calibration vials to resuspend the microspheres Wrong lot number or target values are entered in Update CAL T...

Страница 49: ...are evident on the report contact Technical Support Zero events collected during calibration There is a problem with fluid levels Check the sheath and waste fluid levels Verify that tubing for both b...

Страница 50: ...microspheres have been diluted Do not dilute the control microspheres The sample probe height is incorrect Adjust the sample probe height See page 18 The sample probe is clogged Clean the sample probe...

Страница 51: ...for the calibration microspheres Ensure that the correct wells are selected in the Maintenance tab The calibration lot number or target values selected in setup is incorrect Enter the correct calibrat...

Страница 52: ...problem is analyzer related If Calibration and Controls are successful contact the kit manufacturer A normal bead detail display is shown below It depicts a tight bead population within a white region...

Страница 53: ...il above looks like this Figure 11 Normal Histogram Symptom Possible problem Solution xMAP microspheres classify too high You may be using photobleached calibration microspheres Replace the calibratio...

Страница 54: ...sure there is sheath fluid in the sheath container Prime the system until all air is out of the system Microspheres appear as a long diagonal line The xMAP microspheres have agglutinated Add additiona...

Страница 55: ...Luminex Technical Support Draining the Reservoir If you need to ship the Luminex SD system back to Luminex Corporation drain the reservoir before you pack the system 1 Perform a warmup to pressurize...

Страница 56: ...Luminex 200 System User Manual 46...

Страница 57: ...Fast Acting Fuse Qty 10 CN 0019 01 3 Amp 250 Volts Fast Acting Fuse CN 0051 01 Heater Block XYP CN 0017 01 Luminex 200 System with IS 2 3 LX200 IS2 3 Luminex 200 System with IS 2 3 Developer s Workben...

Страница 58: ...servoir XYP CN 0022 01 Sample Needle Height Alignment Kit Sample Probe Alignment Kit CN 0015 01 Short Sample Needle CN 0006 01 Long Sample Needle CN 0007 01 Sample Holder Large 1 5 mL CN 0008 01 Sampl...

Страница 59: ...ontrol xMAP Classification Control L100 CON1 Microspheres LX100 RP1 Control xMAP Reporter Control L100 CON2 Microspheres MagPlex DD CL1 CL2 Calibrator 5mL MagPlex Classification Calibrator Microsphere...

Страница 60: ...Luminex 200 System User Manual 50...

Страница 61: ...g beam into people s eyes 4 staring into beam 4 B barcode label 13 biohazard 2 biological 1 warning 5 blue indicator light 1 5 C calibration 21 capacity specifications 10 caution 2 CD supplied 9 chemi...

Страница 62: ...pecifications 12 M maintenance schedule annual 27 as required 28 daily 17 monthly 21 routine tasks 19 semi annual 22 weekly 20 manual adjustment 16 mechanical 1 5 monitor waste fluid refill sheath con...

Страница 63: ...yzer 11 Luminex 100 IS 9 11 13 Luminex XYP 12 PC 12 reporter laser 11 sensitivity 10 speed 9 vortex 13 speed specifications 9 start up procedure 17 status of instrument 5 subsystems 13 surge protector...

Страница 64: ......

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