Luminex 200 System User Manual
38
Calibration and
Control Problems
Sample arm does not go down
smoothly.
The 96-well plate is incorrectly
seated in the XYP instrument.
Adjust the 96-well plate.
The 96-well plate is warped.
Inspect the 96-well plate.
Replace it if it is warped.
The sample arm is misaligned.
Readjust the sample arm
horizontal alignment.
The sample probe is bent.
Remove the sample probe from
the Luminex 200 analyzer. Roll
it on a flat surface. If it does not
roll smoothly, replace it with a
new sample probe. Adjust the
sample probe height (see
page 18).
Symptom
Possible problem
Solution
Calibration is slow or fails.
The calibration microspheres
are not fully suspended.
Vortex the calibration vials to
resuspend the microspheres.
Wrong lot number or target
values are entered in
Update
CAL Targets
dialog box.
Verify that correct lot number
and target values are used.
The system calibrators are in the
wrong well on the plate.
Verify that calibrators are in the
correct well.
Not enough calibrator
microspheres added to the well.
Add at least five drops of
calibrator microspheres to the
well. Hold the vial upside down
at a 90 degree angle to the plate
while dispensing them.
Calibrator lot is expired.
Use a fresh bottle of calibrator
microspheres.
The sample probe height is
incorrect.
Adjust the sample probe height.
See page 18.
The sample probe is clogged.
Clean the sample probe. See
page 20.
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