LI-COR ODYSSEY Sa, Руководство оператора

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Odyssey® Sa Imager
Scanning Gels in the Membrane Carrier
The Odyssey Sa Imager can also be used to document Coomassie- stained gels, provided the gels fit
in the Odyssey Sa Membrane Carrier (10.5 x 6.5 cm maximum) and are thin enough (< 2 mm) that
the laser/microscope has enough focus offset to focus in the gel. Maximum focus offset is 3.95 mm
and the focus offset for a given gel is 3.0 plus half the thickness of the gel. Scanning Coomassie-
stained protein gels is described in the Western Blot Analysis protocol found in the Odyssey Sa
Imager Application Protocols manual.
Other protocols developed for the Odyssey Infrared Imaging System, such as In-Gel Westerns, may
be adaptable to the Odyssey Sa Imager, though not currently supported. Factors limiting the
adaptability of these protocols include the size of the gel or membrane versus the Odyssey Sa
Membrane Carrier and the focus offset of the Odyssey Sa Imager (there may not beenough focus
offset to focus in the center of some gels). If you would like to try to adapt one of these protocols,
they can be found at licor.com/support.
Using the Bar Code Reader (Optional Accessory)
When a scan is started, the Odyssey Sa bar code reader automatically scans the side of the microplate
for a bar code. If a compatible bar code is found, it is read and stored in memory. To be compatible,
the characters used in the bar code must be acceptable as file name characters. These include
numerals, upper- and lower-case alphabet, space, underscore, and period. The Image Studio software
can include bar codes in scan names, report names, reports, and data files. Supported bar code
symbologies are Code 39, Codabar, Code 128, and Code 93. Extended Code 39 is not supported. Do
not use optional check characters in the bar codes.
Figure 6-1. Place the bar code on the left side of the plate within the red area, making sure the bars extend above
and below.
The Odyssey Sa bar code reader scans the side of the plate next to column 1 (left side as you face the
instrument). The area that will be read is a 60.3 mm wide strip centered on the edge of the
microplate. The lines of the bar code must extend above and below this 5.0 mm high strip that is
6.4 mm from the bottom of the plate. The minimum bar code line width allowed is 0.177 mm.
Low-profile plates that are at least 10 mm high may also be used. Place the bar code at the top edge
of the plate within the red area.
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