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| LABNET PRISM MICROCENTRIFUGE
6.2 Rotor Maintenance
The rotor should be cleaned thoroughly after each use.
Thorough cleaning must be performed when
spinning samples containing phenol or phenol chloroform.
Periodically inspect the rotor for dents,
dings, scratches, discoloration and cracks. If any damage to the rotor is found, discontinue use of the
rotor immediately and replace.
6.3 Removing and Installing the Angle Rotor
The Prism™ Microcentrifuge comes complete with a standard 24-place rotor installed. To remove
the rotor for cleaning, remove the rotor securing screw from the motor shaft by turning the screw
counterclockwise, using the rotor wrench. Lift the rotor directly upward in a straight vertical motion.
Figure 1. Loading the rotor to achieve balance
To replace rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on
the motor shaft. Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the
rotor with one hand and
tighten the rotor securing screw, using the rotor wrench
.
6.4 Loading the Rotor
Tubes to be loaded should be filled equally by eye. The difference in the weight between the tubes
should not exceed 0.1 gram. Tubes should always be loaded so that there is equal spacing between all
tubes. One or two additional loaded tubes may need to be added to achieve this. Refer to Figure 1. to see
one typical balancing scheme.
6.5 Overloading Rotors
The maximum load of the rotor and maximum speed has been established by the manufacturer. Do
not attempt to exceed these values. The maximum speed of the rotor has been established for liquids
having a homogeneous density of 1.2g/ml or less. In order to centrifuge liquids with a higher density
it is necessary to reduce the speed.
Failure to reduce the speed may result in damage to the rotor and
centrifuge
. The revised maximum speed can be calculated with the following formula:
1.2
higher density value
Reduced speed (n
red
) = × max speed (n
max
)
1.2
1.7
(n
red
) = × 15,000 = 12,602 rpm
Example:
Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:
1.2
higher density value
Reduced speed (n
red
) = × max speed (n
max
)
1.2
1.7
(n
red
) = × 15,000 = 12,602 rpm
If in doubt concerning maximum speeds, please contact the manufacturer for assistance.
