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Operation
BlueShadow Pump 40P Instructions V7613
5.1.2 Isocratic operating mode
Analysis without gradients
The solvent composition is constant during the analysis.
The solvent can be recycled.
5.1.3 Optimizing HPLC separations
To make your HPLC separations as efficient as possible, pay close atten-
tion to the following:
Task
Explanation
Avoiding additional
dead volumes
Once they have been used, never re-use ca-
pillaries in other areas of the HPLC system.
Only use a given PEEK fitting for one specific
port and never re-use it for other ports. Al-
ways install new PEEK fittings on each sepa-
rate port.
Using special
columns
Use special columns and follow the manufactu-
rer‘s instructions on caring for the columns.
Checking for
clogged capillaries
Regularly check for clogged capillaries – test
back pressure without column!
Using filtered
solvents
Use ultra-pure, filtered solvents – Gradient
grade – for the HPLC.
Filtration of substances to be analyzed.
Use of inline filters.
5.2 Purging the pump
Before the pump can be used, it must be purged to remove excess air in
the capillaries.
Flush the pump in the following cases:
At initial startup to eliminate air bubbles in hoses and capillaries.
When changing solvents.
After using buffer solutions to eliminate salt residues.
Before turning off, if you do not plan to restart the device within
shortly.
The installation has been completed.
The capillaries and tubings have been connected.
When is purging
required?
Prerequisites
Column defect
Damage to the column due to purging.
Open the venting screw.
Remove the column.
