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Pump 40P

Instructions

Document no. V7613

HPLC

HPLC

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Содержание BlueShadow 40P

Страница 1: ...Pump 40P Instructions Document no V7613 HPLC HPLC...

Страница 2: ...CET Fax 49 30 8015010 Email support knauer net KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 Internet www knauer net E Mail info knau...

Страница 3: ...eeable misuse 3 2 2 User qualification 3 2 3 Operator responsibility 4 2 4 Personal safety equipment 4 2 5 Working with solvents 5 2 5 1 General requirements 5 2 5 2 Contamination by health threatenin...

Страница 4: ...4 6 3 Connecting devices with LAN 19 4 6 4 Configuring the router 20 4 6 5 Integrating LAN into the company network 21 4 6 6 Controlling several systems separately in LAN 21 4 7 Setting IP addresses...

Страница 5: ...LAN 41 7 2 Possible problems and solutions 42 7 3 System messages 43 8 Maintenance and care 46 8 1 Maintenance contract 46 8 2 Cleaning and caring for the device 46 8 3 Checking the fittings 46 8 4 F...

Страница 6: ...ents and other operating materials 57 11 Technical data 58 11 1 Solvent delivery 58 11 2 Communication 59 11 3 Technical parameters 59 11 4 General 59 12 Chemical compatibility of wetted materials 60...

Страница 7: ...d other instructions from the KNAUER web site www knauer net library 1 2 Signal words Possible dangers related to the device are distinguished in personal and material damages Symbol Meaning DANGER re...

Страница 8: ...ase you notice any damage inform the Technical Support and the shipping company within three workdays 1 4 3 Warranty conditions For information on warranty please refer to our general terms and condi...

Страница 9: ...functionality and safety of the device Operation in potentially explosive areas without special and additio nal explosion protection Contact the KNAUER Customer Support for more information 2 2 User...

Страница 10: ...arly regulate and specify responsibilities for installation operation troubleshooting maintenance and cleaning and set clear rules Ensure that all personnel who work with the device have read and un d...

Страница 11: ...onto hot surfaces in the interior Degas solvents before use and observe their purity 2 5 2 Contamination by health threatening solvents Contamination with toxic infectious or radioactive substances po...

Страница 12: ...n wet rooms 2 7 Maintenance care and repair Avoiding electric shock Before performing any maintenance and ser vice work disconnect the device from the power supply Tools Use only tools recommended or...

Страница 13: ...est form and decontamination report Devices which are shipped without the completed document Service request form and decontamination report will not be repaired If you would like to return a device t...

Страница 14: ...rew 4Keypad 5Tube connections for the piston backflushing 6Pump head Fig 1 Front view 3 1 2 Rear view Legend 1Warranty seal 2Space for rear label with serial number 3Fan opening 4Warning 1 5Power swit...

Страница 15: ...on made of stainless steel Ceramic inlays for biocompatible applications The front of the pump head is labeled with the max pumping capacity 10 ml or 50 ml Pump heads with inlays carry additional mate...

Страница 16: ...he product spe cific requirements of European directives This is confirmed in a Declaration of Conformity A warranty seal is affixed to some devices For more information see chap 1 4 4 on p 2 3 6 Scop...

Страница 17: ...front cover or leak tray as these parts are loosely attached to the device Keep the included packing list for repeat orders Keep the original packaging for safe storage or transportation 4 2 Ambient c...

Страница 18: ...briefly when the device is switched on Main connection The electrical power supply at the operation site must be connected directly to the nearest main power connection Grounding The connectors for th...

Страница 19: ...one device through another the pin header is used To use remote control you have to connect cables to the pin header The single ports are used to exchange control signals The device is switched off Th...

Страница 20: ...opening 2 below the inserted operating tool 3 Remove the depressor tool Check if the cables are firmly connected Push the terminal strip onto the pin header Finish the installation Put the device int...

Страница 21: ...hen the pump starts START IN Device starts after signal short circuit to GND of an external device During software control an electronic trigger is sent via LAN ERROR OUT Output is active until the Er...

Страница 22: ...irection of the fitting Fig 6 Setup of a flangeless fitting 4 Manually fasten the flangeless fitting 4 to the device Fig 7 Fully assembled flangeless fitting 4 5 2 Connecting the pump head to the solv...

Страница 23: ...s V7613 Procedure Process Figure 1 Slide the flangeless fitting over the tubing 2 Insert the tubing into the free inlet 1 on the bottom of the pump head 3 Tighten the fitting by hand Fig 8 Solvent lin...

Страница 24: ...ctors on rear side Note HPLC devices from KNAUER only work with IP addresses which have been assigned by IPv4 IPv6 is not supported This chapter describes how to set up a chromatography system in a lo...

Страница 25: ...all LAN devices Disable the setting for the network adapter in Device Manager Allow the computer to turn off this device to save power Process 1 In Windows open Network and Sharing Center 2 Double cli...

Страница 26: ...r 3 Use the power supply to connect the router to the mains power system Set the router properties see chapter 4 6 4 on page 20 4 6 4 Configuring the router The router is preset at the factory Informa...

Страница 27: ...ference Therefore the port number for each device must be changed and this same number must be entered into the device configuration of the chromatography software We recom mend to use the same port n...

Страница 28: ...e Wizard and the device has been established Process 1 In Firmware Wizard click Reset LAN Settings 2 The window Device connection settings opens Enter serial number of the device into the text field T...

Страница 29: ...he window Device connection settings opens Enter serial number of the device into the text field Target device serial num ber 1 3 Select option Obtain an IP address automatically 2 4 Click Reset Conn...

Страница 30: ...ke sure to connect the correct solvent as described in the specification table found in the supplement Running In procedure for pump heads V6894 If a pump was not in operation for a long time e g afte...

Страница 31: ...instructions on caring for the columns Checking for clogged capillaries Regularly check for clogged capillaries test back pressure without column Using filtered solvents Use ultra pure filtered solven...

Страница 32: ...ig 12 Venting screw of the pres sure sensor The purging process of the pump is limited to a maximum pressure of 5 MPa If this value is exceeded during the purging process the pump switches off automat...

Страница 33: ...backflushing 5 3 Switch on and self test 1 Switch on the pump 2 The display shows Initialization The device runs a self test 3 Wait until the self test has been completed 4 After the test has been su...

Страница 34: ...LED on the front side The co lor of the LED shows the current status LED Status Green Pump is ready idle status Green flashing Pump is running Yellow Firmware upload in progress Yellow flashing Devic...

Страница 35: ...avigation button Navigation through menu with horizontal keys Adjustment of values at input fields with verti cal keys Menu button Select main menu Return to one menu further up in the menu structure...

Страница 36: ...d Link menus can be accessed by using the menu button Note If the status PLAY is displayed on the screen the pump is not running By pushing the pump starts running If STOP is displayed the pump is run...

Страница 37: ...31 Operation BlueShadow Pump 40P Instructions V7613 5 5 2 Menu structure overview Fig 15 Menu structure overview...

Страница 38: ...for external commu nication the device should be restarted Pr limits Settings for the minimum and maximum pressure The pump stops automatically if the pres sure drops below the minimum value or exceed...

Страница 39: ...46 Water methanol 80 20 0 40 Water methanol 90 10 0 34 Start input After receiving a signal from an external device the device or a program start an automatic run Software sends a trigger signal via L...

Страница 40: ...for signal smoothing The larger the value of the selected time constant the stronger the applied signal smoothing Analog input setup Note Before operating this con figuration change to the Net work Co...

Страница 41: ...u 2 If no buttons are pressed the display returns to the main menu after a short time 3 The menu contains no editable parameters 5 7 Program menu The menu Program is used to list and create programs T...

Страница 42: ...ow on the lower left side of the display and press the downward key to create a new line 5 Enter the time value of the new line and adjust the respective parameters 6 Press for two seconds to exit the...

Страница 43: ...ate the numeric pad by pressing and enter the program number you wish to delete 4 You will be asked whether you really want to delete the program Confirm with the confirm key or deny with 5 7 6 Create...

Страница 44: ...line to navigate to the next line or create a new line 5 8 1 Create link Process Figure 1 Select the Link menu 2 Enter the link number 3 Press to enter the editing mode of the link 4 Move the cursor t...

Страница 45: ...ng with the initial flow rate of the program 5 Navigate to the pump status and press the up arrow key to set the pump into a run status RUN 6 The link will start running the programs 7 Use the down ar...

Страница 46: ...on OQ The Operation Qualification includes an extensive functionality test accor ding to KNAUER standard OQ documents The Operation Qualification is a standardized document and free of charge It is no...

Страница 47: ...set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connection...

Страница 48: ...flushing inform the technical support Pump head maintenance is required Pressure and flow rate variations Purge the pump head to remove the air bub bles Always tighten the inlet screw fittings and out...

Страница 49: ...een installed C Cannot delete active program link Pause active program as well as Link and the delete the link Cannot edit program from the running link First pause the link then edit the data on the...

Страница 50: ...en completed then change the link or delete it M Maximum pressure System stopped Reduce the pressure or adjust the upper pressure limit Restart the system Minimum pressure System stopped Increase the...

Страница 51: ...edit or delete link This program is used in a link First pause or delete link then edit or delete data by means of the chromatography software This program is used in WakeUp First quit or delete wake...

Страница 52: ...e Pull the power plug Users may perform the following maintenance tasks themselves Replacing the pump head Replacing the check valves of the pump head 8 2 Cleaning and caring for the device All smooth...

Страница 53: ...nd to remove air bubbles in the capillary and tubing If buffer so lution has been employed note that the buffer solution and the flushing solution are compatible When buffers have been used flush with...

Страница 54: ...ush the pump head before changing Device defect Residuals chemicals can damage the pump head if they are not being removed before storage For reuse flush the pump head and fill it with isopropanol Bef...

Страница 55: ...size 1 4 Remove the capillary Fig 17 Outlet screw fitting 4 Disconnect the tubing of the piston backflushing 1 from the flush pump and the pump head Fig 18 Tubings piston backflushing 5 Using the All...

Страница 56: ...re Process Figure 1 Unscrew and remove the capil lary connector 1 2 Loosen the outlet fitting 2 with the open end wrench 3 Remove the first check valve 4 Loosen the inlet fitting 3 with the open end w...

Страница 57: ...k valves in the direction of the flow The notch of the check valve points downward Insert the check valves in the direction of the flow The arrow on the check valve points upward The check valves have...

Страница 58: ...and unscrew it by hand 3 Insert a new filter cartridge 8 6 1 Removing filter cartridge The screw connection of the filter cartridge is located above the pressure sensor The pump has been purged Open...

Страница 59: ...dge A96601 Fig 27 Filter cartridge A9661 Filter cartridges have a marking of the flow direction The filter cartridge with article number A96601 stainless steel is inserted into the pressure sensor in...

Страница 60: ...A96601 2 Screw the screw connection 2by hand into the pressure sensor 3 Tighten the screw connec tions with the torque wrench to the appropriate torque Fig 29 Tighten screw connection Component defec...

Страница 61: ...ransporting the device Carefully prepare the device for transport or storage If you want to return your device to KNAUER for repairs enclose the Service Request Form which can be downloaded from our w...

Страница 62: ...Storing the pump head If you want to store pump heads separately proceed as follows The pump head has been dismounted see chapter 8 5 1 on page 48 Syringe Flushing solution Procedure Process 1 Fill th...

Страница 63: ...mber classifies to category 8 and 9 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive En...

Страница 64: ...40 ml min recommended 0 1 20 ml min continuous operation Maximum pressure 10 ml pump head Stainless steel 65 MPa up to 5 ml min 40 MPa up to 10 ml min Ceramic 40 MPa Maximum pressure 50 ml pump head...

Страница 65: ...is detected automatically using radio frequency identification RFID GLP RFID pump head recognition detailed report Display Keypad 11 4 General Supply voltage range 100 240 V Supply frequency 50 60 Hz...

Страница 66: ...rwise Please note that higher temperatures can signifi cantly affect the stability of different materials 12 2 Plastics Polyetheretherketone PEEK PEEK is a durable and resistant plastic and next to st...

Страница 67: ...e POM POM H TF POM is a semi crystalline high molecular thermoplastic material which stands out due to its high stiffness low friction value and thermic stability It can even substitute metal in many...

Страница 68: ...ized by a high hardness a low coefficient of fric tion and thus low wear In addition it is highly biocompatible DLC is inert against all acids alkalis and solvents commonly used in HPLC Ceramic Cerami...

Страница 69: ...used or with a mixture of higher compatibility They are inert against almost all solvents Exceptions are biological appli cations which are metal ion sensible and applications with extreme cor rosive...

Страница 70: ...APC40FA Pump with 50 ml pump head ceramic with accessories APC60FB Pump with 50 ml pump head stainless steel for high temperature applications with accessories APC40FE Pump with 50 ml pump head stainl...

Страница 71: ...AHC22 50 ml pump head ceramic for water applicati ons with accessories AHC22FA Check valve for 10 ml and 50 ml pump head for dosing applications A06840 Check valve for 10 ml pump head for HPLC applica...

Страница 72: ...UER instructions online www knauer net library KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KN...

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