13
6-4. Setting the sampling amount
Put the sample in the sample cell selected in section 6-1 and determine the sampling amount.
* If the sampling amount (sample height) is not consistent, it may affect the absorbance.
* When not using the light shielding cover, determine the sampling amount under the same condition of ex-
ternal light as measurement.
* Use the same amount in making calibration curve and measurement.
①
Set the absorbance measurement mode.
Enter the absorbance measurement mode by pressing the [MODE] key.
(
→
8. Measurement (P.19))
Immediately before performing this operation, it is recommended to perform zero-adjustment.
(
→
9. Zero-adjustment (P.20))
0 1
X 1
0 . 0 0 0 1
X 2
0 . 0 0 0 2
②
Measure the absorbance.
Measure the absorbance after setting the sample to the sample cell selected in the previous
section 6-1.
0 1
X 1
0 . 9 3 4 0
X 2
0 . 7 5 0 8
③
Observe the change of absorbance.
As the sample is gradually raised, the fluctuation of displayed absorbance, X1 and X2, will be-
come stable at a point where the near-infrared pass-through is stopped. Consider the sampling
amount at this point as "absorbance saturated sampling amount". If the sampling amount is
sufficiently prepared, set the sampling amount more than the "absorbance saturated sampling
amount".
Sampling
amount
Sampling
amount
Sampling
amount
Near-infrared
Near-infrared
Sampling
amount
Absorbance
* Refer to P.29, if the absorbance saturated sampling amount cannot be prepared.
When the sample height is raised, there will be a point
where the near-infrared pass-through is stopped.
Then, the value will become stable. Consider the
point where only the last 2 digits of absorbance fluc-
tuate as the absorbance saturated sampling amount.
X 1
1 . 0 3
4 5 . . . . .
1 . 0 3
5 7
X 2
0 . 9 5
3 1 . . . . .
0 . 9 5
2 3
While the near-infrared from the bottom passes
through the sample, the displayed value will contin-
ue to fluctuate.
X 1 0 . 8 7 6 5 . . . . . 0 . 9 9 8 7 . . . . 1 . 0 0 1 5
X 2 0 . 7 8 9 0 . . . . . 0 . 8 3 4 5 . . . . 0 . 9 3 7 6
Ex:
Ex:
No more change in raising the height of sample
